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MAB2259 Anti-Cholesterol-24 Hydroxylase Antibody, clone1A7

Detect Cholesterol-24 Hydroxylase using this Anti-Cholesterol-24 Hydroxylase Antibody, clone1A7 validated for use in WB, IP, IC, IH.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Signaling Neuroscience Gene Symbol: CH24H, CP46, CYP46, CYP46A1 UniProt Number: Q9Y6A2
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MAB2259
100 µg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, H, R, RbWB, IP, ICC, IHCMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMAB2259
      DescriptionAnti-Cholesterol-24 Hydroxylase Antibody, clone1A7
      Alternate Names
      • Cytochrome P450 46A1
      • cholesterol 24-hydroxylase
      • cytochrome P450, family 46
      • cytochrome P450, family 46, subfamily A, polypeptide 1
      • cytochrome P450, subfamily 46 (cholesterol 24-hydroxylase)
      Background InformationIn enzymology, a cholesterol 24-hydroxylase (EC 1.14.13.98) is an enzyme that catalyzes the chemical reaction:
      cholesterol + NADPH + H+ + O2 --> (24S)-24-hydroxycholesterol + NADP+ + H2O. Cholesterol 24-hydroxylase, a cytochrome P450 (CYP46A1), is expressed at 100-fold higher levels in the brain than in the liver. This enzyme belongs to the family of oxidoreductases, specifically those acting on paired donors, with O2 as oxidant and incorporation or reduction of oxygen. Studies indicate that cholesterol 24-hydroxylase constitutes a major tissue-specific pathway for cholesterol turnover in the brain (Lund, 2003).
      References
      Product Information
      FormatPurified
      Control
      • Mouse brain lysate
      PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationDetect Cholesterol-24 Hydroxylase using this Anti-Cholesterol-24 Hydroxylase Antibody, clone1A7 validated for use in WB, IP, IC, IH.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      • Immunocytochemistry
      • Immunohistochemistry
      Biological Information
      ImmunogenHis-tagged recombinant protein corresponding to human cholesterol-24 hydroxylase.
      Epitopeunknown
      Clone1A7
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThis antibody recognizes cholesterol-24 hydroxylase.
      IsotypeIgG1κ
      Species Reactivity
      • Mouse
      • Human
      • Rat
      • Rabbit
      Species Reactivity NoteDemonstrated to react with mouse. Predicted to react with human, rat, and rabbit based on 100% sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum protein is expressed in the brain, where it converts cholesterol to 24S-hydroxycholesterol. While cholesterol cannot pass the blood-brain barrier, 24S-hydroxycholesterol can be secreted in the brain into the circulation to be returned to the liver for catabolism. [provided by RefSeq].
      Gene Symbol
      • CH24H
      • CP46
      • CYP46
      • CYP46A1
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: Involved in the turnover of cholesterol. It converts cholesterol into 24S-hydroxycholesterol and, to a lesser extent, 25-hydroxycholesterol.

      CATALYTIC ACTIVITY: Cholesterol + NADPH + O2 = (24S)-24-hydroxycholesterol + NADP+ + H2O.

      COFACTOR: Heme group By similarity.

      SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Single-pass membrane protein. Microsome membrane; Single-pass membrane protein.

      TISSUE SPECIFICTY: Expressed in brain. The mRNA was broadly distributed with higher levels in gray matter zones and lower levels in regions rich in white matter. Not detected in fetal sample but its expression increases linearly with age.

      SEQUENCE SIMILARITIES: Belongs to the cytochrome P450 family.

      BIOPHYSICOCHEMICAL PROPERTIEIS: Kinetic parameters:

      KM=2.2 µM for 24(S)-hydroxycholesterol
      Molecular Weight~ 54 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in Cholesterol-24 hydroxylase mouse brain lysate.

      Western Blot Analysis: 1 µg/ml of this antibody detected Cholesterol-24 hydroxylase on 10 µg of Cholesterol-24 hydroxylase mouse brain lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MAB2259 04053252467332

      Documentation

      Anti-Cholesterol-24 Hydroxylase Antibody, clone1A7 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Cholesterol-24 Hydroxylase Antibody, clone1A7 Certificates of Analysis

      TitleLot Number
      Anti-Cholesterol-24 Hydroxylase, clone 1A7 - 2443060 2443060
      Anti-Cholesterol-24 Hydroxylase, clone 1A7 - 2006954 2006954
      Anti-Cholesterol-24 Hydroxylase, clone 1A7 - 2021742 2021742
      Anti-Cholesterol-24 Hydroxylase, clone 1A7 - 2197287 2197287
      Anti-Cholesterol-24 Hydroxylase, clone 1A7 - 3185040 3185040
      Anti-Cholesterol-24 Hydroxylase, clone 1A7 - 3189711 3189711
      Anti-Cholesterol-24 Hydroxylase, clone 1A7 - 3281613 3281613
      Anti-Cholesterol-24 Hydroxylase, clone 1A7 - 3682587 3682587
      Anti-Cholesterol-24 Hydroxylase, clone 1A7 - 3719621 3719621
      Anti-Cholesterol-24 Hydroxylase, clone 1A7 - 3789406 3789406
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