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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
polyclonal
Application:
ICC, IHC, WB
Citations:
20
biological source
rabbit
Quality Segment
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
polyclonal
species reactivity
pig, human, mouse, Xenopus, bovine, rat
manufacturer/tradename
Upstate®
technique(s)
immunocytochemistry: suitable, immunohistochemistry: suitable, western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... CALR(811)
General description
60kDa
Immunogen
peptide (QAKDEL) corresponding to the C-terminus (amino acids 412-417) of human calreticulin
Application
Anti-Calreticulin Antibody is an antibody against Calreticulin for use in IC, IH & WB.
Research Sub Category
PI3K, Akt, & mTOR Signaling
Enzymes & Biochemistry
PI3K, Akt, & mTOR Signaling
Enzymes & Biochemistry
Biochem/physiol Actions
calreticulin; does not cross react with other endoplasmic reticulum proteins
Physical form
0.1M Tris-glycine, pH 7.0, 0.15M NaCl and 0.05% sodium azide.
Format: Purified
Analysis Note
Control
Positive Antigen Control: Catalog #12-305, 3T3/A31 lysate. Add 2.5 μL of 2-mercapto-ethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.
Positive Antigen Control: Catalog #12-305, 3T3/A31 lysate. Add 2.5 μL of 2-mercapto-ethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.
routinely evaluated by immunoblot on RIPA lysates from murine 3T3/NIH fibroblasts
Other Notes
Replaces: MABT145
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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