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CBL496 Anti-CD34 Class II Antibody, clone QBEND/10

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CBL496
100 µg  
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      Overview

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MkFC, IP, IHC, IH(P)MPurifiedMonoclonal Antibody
      Description
      Catalogue NumberCBL496
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-CD34 Class II Antibody, clone QBEND/10
      Product Information
      FormatPurified
      PresentationThe monoclonal is presented at a concentration of 100μg/1ml in phosphate buffered saline containing 10mM sodium azide and 1mg/ml bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application.
      Quality LevelMQ100
      Applications
      ApplicationAnti-CD34 Antibody, Class II, clone QBEND/10 is an antibody against CD34 Class II for use in FC, IP, IH, IH(P).
      Key Applications
      • Flow Cytometry
      • Immunoprecipitation
      • Immunohistochemistry
      • Immunohistochemistry (Paraffin)
      Application NotesIdentification of haemopoietic progenitor cells

      Studies into the control mechanisms of early haemopoiesis

      Purification of haemopoietic cells from, for example, FACS

      The antibody reacts with both frozen and fixed tissue sections from vascular endothelium and stromal fibroblasts

      Immunohistochemistry in parraffin sections: Pretreatment is HIER with citrate buffer, pH 6.0.

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenHuman endothelial vesicles
      CloneQBEND/10
      HostMouse
      SpecificityThe antibody recognizes a heavily glycosylated transmembrane protein: gp 105-120 kDa. The antigen is expressed on immature human haemopoietic precursor cells, capillary endothelial cells and human myeloid cells in the following categories: subsets of CFU-GEMM and BFU-E, all CFU-GM, granulocyte precursors, monocytes, myeloid leukaemias. This antibody recognizes the class II epitope showing resistance to neuraminidase enzyme cleavage.

      KNOWN SPECIES CROSS REACTIVITY: Positive with rhesus monkey peripheral blood leucocytes in flow cytometry.

      FUSION PARTNER: NSO myeloma cell line
      IsotypeIgG1
      Species Reactivity
      • Human
      • Monkey
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryCD34 is a monomeric cell surface antigen with a molecular mass of approximately 110 kD that is selectively expressed on human hematopoietic progenitor cells.[supplied by OMIM]
      Gene Symbol
      • CD34
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P28906 # Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins.
      SIZE: 385 amino acids; 40716 Da
      SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
      TISSUE SPECIFICITY: Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues.
      DEVELOPMENTAL STAGE: On early hematopoietic progenitor cells.
      PTM: Highly glycosylated. & Phosphorylated on serine residues by PKC.
      DISEASE:SwissProt: P28906 # Abnormal CD34 expression in leukemogenesis.
      SIMILARITY: SwissProt: P28906 ## Belongs to the CD34 family.
      Product Usage Statements
      Availability by Geography
      • This product is not available for sale in the United States.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsFor use within 1 month of purchase store at +4°C, for long term storage aliquot antibody into small volumes and store at -20°C.
      Packaging Information
      Material Size100 µg
      Global Trade Item Number
      Catalogue Number GTIN
      CBL496 04053252671210