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MABT84 Anti-Aggrecan Antibody, clone 1-C-6

This Anti-Aggrecan Antibody, clone 1-C-6 is validated for use in IH, WB for the detection of Aggrecan.

Anti-Aggrecan Antibody, clone 1-C-6 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Research Sub-Category: ECM Proteins Gene Symbol: Acan, Agc, Agc1 UniProt Number: P07897
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MABT84
100 µg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      R, M, BIHC, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABT84
      DescriptionAnti-Aggrecan Antibody, clone 1-C-6
      Alternate Names
      • Aggrecan core protein
      • Cartilage-specific proteoglycan core protein
      • CSPCP
      Background InformationAggregating chondroitin sulfate proteoglycan or aggrecan, is one of the most studied proteoglycans. Many individual monomers of aggrecan bind to hyaluronic acid to form an aggregate, the monomer is known as aggrecan. The aggregates are comprised of up to 100 monomers attached to a single chain of hyaluronic acid. The high content of negatively charged polysaccharide chains contributes to the osmotic swelling of cartilage and in combination with intact collagen fibers results in articular cartilage resistance to compressive forces and high tensile strength. Aggrecan is abundant in articular cartilage and compromises 10% of its dry weight.
      Product Information
      FormatPurified
      Control
      • Normal mouse cartilage and normal rat cartilage tissues
      PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-Aggrecan Antibody, clone 1-C-6 is validated for use in IH, WB for the detection of Aggrecan.
      Key Applications
      • Immunohistochemistry
      • Western Blotting
      Application NotesWestern Blot Analysis: 1 µg/mL from a representative lot detected Aggrecan on 10 µg of A1D4 protein fraction from Bovine tissue.
      Biological Information
      ImmunogenReduced and alkylated Aggrecan isolated from Swarm Rat Chondrosarcoma
      Clone1-C-6
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      IsotypeIgG1κ
      Species Reactivity
      • Rat
      • Mouse
      • Bovine
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryAn extracellular matrix proteoglycan; cleaved by ADAMTS1 [RGD, Feb 2006].
      Gene Symbol
      • Acan
      • Agc
      • Agc1
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: This proteoglycan is a major component of extracellular matrix of cartilagenous tissues. A major function of this protein is to resist compression in cartilage. It binds avidly to hyaluronic acid via an N-terminal globular region. May play a regulatory role in the matrix assembly of the cartilage.

      SUBUNIT STRUCTURE: Interacts with FBLN1 and COMP (By similarity).

      SUBCELLULAR LOCATION: Secreted › extracellular space › extracellular matrix (By similarity).

      DOMAIN: Two globular domains, G1 and G2, comprise the N-terminus of the proteoglycan, while another globular region, G3, makes up the C-terminus. G1 contains Link domains and thus consists of three disulfide-bonded loop structures designated as the A, B, B' motifs. G2 is similar to G1. The keratan sulfate (KS) and the chondroitin sulfate (CS) attachment domains lie between G2 and G3.

      PTM: Contains mostly chondroitin sulfate, but also keratan sulfate chains, N-linked and O-linked oligosaccharides.

      SEQUENCE SIMILARITIES: Belongs to the aggrecan/versican proteoglycan family.

      Contains 1 C-type lectin domain.

      Contains 1 Ig-like V-type (immunoglobulin-like) domain.

      Contains 4 Link domains.

      Contains 1 Sushi (CCP/SCR) domain.
      Molecular WeightVarious
      Product Usage Statements
      Quality AssuranceEvaluated by Immunohistochemistry in normal mouse cartilage and normal rat cartilage tissues.

      Immunohistochemistry Analysis: A 1:1,000 dilution of this antibody detected Aggrecan in normal mouse cartilage and normal rat cartilage tissues.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Global Trade Item Number
      Catalogue Number GTIN
      MABT84 04053252633713