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ABC974 Anti-ATG8 Antibody

This Anti-ATG8 Antibody is validated for use in Western Blotting for the detection of ATG8.

Anti-ATG8 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Research Sub-Category: Apoptosis - Additional Gene Symbol: Atg8a, Atg8b UniProt Number: Q9W2S2/Q9VEG5
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ABC974
100 μg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      DrWBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABC974
      DescriptionAnti-ATG8 Antibody
      Alternate Names
      • Atg8
      • Atg8a
      • Atg8b
      • Autophagy-specific gene 8a
      • Autophagy-specific gene 8b
      Background InformationAutophagy-specific gene 8a and 8b (UniProt Q9W2S2 and Q9VEG5, respectively) are encoded by the drosophila Atg8a (ORF Dmel_CG32672; Gene ID 32001) and Atg8b (ORF Dmel_CG12334; Gene ID 42132) gene, respectively. Autophagy is a fundamental an adaptive response to cellular stress caused by, for example, starvation and viral infection. Autophagy is a process conserved from yeast to human and involves the formation of double-membrane vesicles for the purpose of recycling nutrients by means of degrading the engulfed content. In resting cells, the majority of Atg8 is in an unlipidated form called Atg8-I. During autophagy, Atg8-I is conjugated with a lipid moiety, and the lipidated form (Atg8-II) is recruited to autophagosomal membranes. An elevated Atg8-II level therefore serves as an indicator of autophagy activation. LC3 is the mammalian ortholog of drosophila Atg8.
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal antibody in PBS with 0.02% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-ATG8 Antibody is validated for use in Western Blotting for the detection of ATG8.
      Key Applications
      • Western Blotting
      Application NotesWestern Blotting Analysis: A representative lot detected the induction of the lipidated Atg8-II band in wild-type flies upon Rift Valley fever virus (RVFV) infection, while an enhanced Atg8-II induction was observed in Toll-1-overexpressing flies and no Atg8-II induction by RVFV infection was seen among Toll-7 mutant flies (Moy, R.H., et al. (2014). Immunity. 40(1):51-65).
      Western Blotting Analysis: A representative lot detected sdRNA-targeted downregulation of Atg8a and Atg8b in Drosophila S2 cells (Shelly, S., et al. (2009). Immunity. 30(4):588-598).
      Western Blotting Analysis: A representative lot detected the 16 kDa Atg8-I band (unlipidated) as well as the serum starvation- and vesicular stomatitis virus (VSV) infection-induced 14 kDa Atg8-II band (lipidated) in Drosophila S2 cells (Shelly, S., et al. (2009). Immunity. 30(4):588-598).
      Biological Information
      ImmunogenLinear peptide based on drosophila Atg8a and Atg8b sequences near the N-terminus.
      EpitopeNear N-terminus.
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityReacts with the unlipidated (Atg8-I) and lipidated (Atg8-II) forms of drosophila Atg8a and Atg8b (Shelly, S., et al. (2009). Immunity. 30(4):588-598).
      Species Reactivity
      • Drosophila
      Species Reactivity NoteDrosophila. Predicted to react with C. Elegans based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Atg8a
      • Atg8b
      Purification MethodAffinity Purfied
      UniProt Number
      Molecular Weight~16/14 kDa observed. The upper band of the doublet represents the unlipidated Atg8-I form, while the lower band of the doublet represents the lipidated Atg8-II form that is upregulated upon autophagy induction.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in DL1 cell lysate.

      Western Blotting Analysis: 0.5 µg/mL of this antibody detected ATG8 in 10 µg of DL1 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABC974 04055977314892