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AP1144 Anti-ACP1 Mouse mAb (4B10)

Anti-ACP1 Mouse mAb (4B10) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Acid Phosphatase of Erythrocyte

AP1144
  
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      Overview

      Replacement Information

      Key Spec Table

      Host
      M
      Description
      Overview

      This product has been discontinued.





      Recognizes the ~17 kDA ACP1 protein in HeLa, PC-12, and NIH3T3 cells.
      Catalogue NumberAP1144
      Brand Family Calbiochem®
      SynonymsAnti-Acid Phosphatase of Erythrocyte
      Application Data
      Detection of human ACP1 by immunoblotting. Sample: Whole cell lysate from HeLa cells. Primary antibody: Anti-ACP1 Mouse mAb (4B10) (Cat. No. AP1144) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human ACP1 by immunoblotting. Sample: Whole cell lysate from PC-12 cells. Primary antibody: Anti-ACP1 Mouse mAb (4B10) (Cat. No. AP1144) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human ACP1 by immunoblotting. Sample: Whole cell lysate from NIH 3T3 cells. Primary antibody: Anti-ACP1 Mouse mAb (4B10) (Cat. No. AP1144) (1-5 µg/ml). Detection: chemiluminescence.
      References
      Product Information
      FormLiquid
      FormulationIn PBS, pH 7.2.
      Positive controlHeLa cells, PC-12 cells, NIH3T3 cells
      PreservativeNone
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Application NotesELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      ImmunogenFull-length, recombinant ACP1 (aa1-159), expressed as a GST fusion protein
      Clone4B10
      HostMouse
      IsotypeIgG2,b
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Regulatory Review
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      AP1144 0

      Documentation

      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision21-October-2010 JLN
      SynonymsAnti-Acid Phosphatase of Erythrocyte
      ApplicationELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Application Data
      Detection of human ACP1 by immunoblotting. Sample: Whole cell lysate from HeLa cells. Primary antibody: Anti-ACP1 Mouse mAb (4B10) (Cat. No. AP1144) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human ACP1 by immunoblotting. Sample: Whole cell lysate from PC-12 cells. Primary antibody: Anti-ACP1 Mouse mAb (4B10) (Cat. No. AP1144) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human ACP1 by immunoblotting. Sample: Whole cell lysate from NIH 3T3 cells. Primary antibody: Anti-ACP1 Mouse mAb (4B10) (Cat. No. AP1144) (1-5 µg/ml). Detection: chemiluminescence.
      DescriptionA purified mouse monoclonal antibody, which recognizes the ~17 kDA ACP1 protein.
      BackgroundPhosphatase enzymes catalyse hydrolysis of phosphoric acid esters of various alcohols.
      HostMouse
      ImmunogenFull-length, recombinant ACP1 (aa1-159), expressed as a GST fusion protein
      Clone4B10
      IsotypeIgG2,b
      Specieshuman, mouse, rat
      Positive controlHeLa cells, PC-12 cells, NIH3T3 cells
      FormLiquid
      FormulationIn PBS, pH 7.2.
      PreservativeNone
      CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Regulatory Review