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MABS416 Anti-ABCG5, clone 1B5E10 Antibody

Anti-ABCG5, clone 1B5E10 Antibody is an antibody against ABCG5 for use in western blotting, IHC, flow cytometry & ELISA.

Anti-ABCG5, clone 1B5E10 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Metabolic Hormones & Receptors Gene Symbol: ABCG5 UniProt Number: Q9H222
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MABS416
100 µL  
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      Overview

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      100% Performance Guaranteed

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IHC, FC, ELISAMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABS416
      ReplacesMABN1076
      DescriptionAnti-ABCG5, clone 1B5E10 Antibody
      Alternate Names
      • ATP-binding cassette sub-family G member 5
      • Sterolin-1
      • ABCG5
      Background InformationATP-binding cassette sub-family G member 5, also known as Sterolin-1, and encoded by the gene name ABCG5, plays an important role in the selective transport of the dietary cholesterol in and out of the enterocytes and in the selective sterol excretion by the liver into bile. ABCG5 is localized too the membrane and is expressed in the liver, lower levels in the small intestine and colon. Mutations associated with ABCG5 is Sitosterolemia, which is a rare autosomal recessive disorder characterized by increased intestinal absorption of all sterols including cholesterol, plant and shellfish sterols, and decreased biliary excretion of dietary sterols into bile.
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal in PBS containing up to 0.1% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-ABCG5, clone 1B5E10 Antibody is an antibody against ABCG5 for use in western blotting, IHC, flow cytometry & ELISA.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      • Flow Cytometry
      • ELISA
      Application NotesImmunohistochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected ABCG5 in cervical and rectum cancer tissues.

      Flow Cytometry Analysis: A 1:200-400 dilution from a representative lot detected ABCG5 in A549 cells.

      ELISA: A representative lot detected control Antigen (100 ng) and Antigen (10 ng, 50 ng, 100 ng) in ELISA (Direct).
      Biological Information
      ImmunogenPurified recombinant fragment of human ABCG5 expressed in E. Coli
      Clone1B5E10
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      IsotypeIgG1
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • ABCG5
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~70 kDa observed. Uncharacterized bands may be observed in some lysate(s).
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HL7702, Raji and Jurkat cell lysates.

      Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected ABCG5 in HL7702, Raji and Jurkat cell lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Global Trade Item Number
      Catalogue Number GTIN
      MABS416 04053252957796