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NA02 Anti-2,2,7-Trimethylguanosine Mouse mAb (K121)

This Anti-2,2,7-Trimethylguanosine Mouse mAb (K121) is validated for use in Affinity Purification, ICC, IP, Paraffin Sections for the detection of 2,2,7-Trimethylguanosine.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
NA02
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Overview

Key Spec Table

Species ReactivityHostAntibody Type
H, M, RMMonoclonal Antibody

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NA02-100UG
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      Description
      OverviewRecognizes the exposed 5′ cap structure (2,2,7-trimethylguanosine) of small nuclear RNAs (snRNAs).
      Catalogue NumberNA02
      Brand Family Calbiochem®
      Application Data
      Detection of the 5' cap of snRNA by immunocytochemistry. Sample: HeLa cells fixed with methanol. Primary antibody: Anti-2,2,7-Trimethylguanosine Mouse mAb (K121) (Cat. No. NA02) (0.8 μg/ml). Detection: fluorescence.
      References
      ReferencesCasciola-Rosen, L.A., et al. 1994. J. Biol. Chem. 269, 30757-30760.
      Krainer, A.R. 1988. Nuc. Acids Res. 16, 9415.
      Reddy, R., and Busch, H., 1988. Birnstiel, M.L. (ed) Springer-Verlag, Berlin and Heidelberg. 1.
      Krainer, A.R., and Maniatis, T. 1985. Cell 42, 725.
      Kramer, A., et al. 1984. Cell 38, 299.
      Lerner, M.R., et al. 1980. Nature 283, 220.
      Product Information
      FormLiquid
      FormulationIn 0.05 M sodium phosphate buffer, 0.2% gelatin.
      Negative controlTransfer RNA (tRNA)
      Positive controlHeterogenous nuclear RNA (hnRNA)
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Application ReferencesAffinity Purification Luhrmann, R. 1988. Birntiel, M.L. (ed) Springer-Verlag, Berlin and Heidelberg. 71. Immunoprecipitation Moteki, S. and Price, D. 2002. Mol. Cell 10, 599. Paraffin Sections Volm, M., et al. 2000. Clin. Cancer Res. 6, 3236.
      Key Applications Immunocytochemistry
      Immunoprecipitation
      Paraffin Sections
      Application NotesAffinity Purification (use Cat. No. NA02A, see application references)
      Immunocytochemistry (0.5-5 µg/ml)
      Immunoprecipitation (1-2 µg, see application references)
      Paraffin Sections (see application references)
      Application CommentsAlso recognizes 7-methylguanosine-capped mRNA. This antibody reacts with the exposed 5' cap structure of the snRNAs and the antibody can be used for the purification of snRNPs by immunoaffinity chromatography (use Cat. No. NA02A). All of the major snRNPs bind to the monoclonal antibody column and can be gently eluted by washing with 7-methyl guanosine. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogen2,2,7-trimethylguanosine
      CloneK121
      HostMouse
      IsotypeIgG₁
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Yes
      Global Trade Item Number
      Catalogue Number GTIN
      NA02-100UG 07790788053840