Amicon® Ultra-15 Centrifugal Filter Units
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Overview
- High recovery Ultracel regenerated cellulose membrane in a range of molecular weight cut-offs
- High retentate recovery of >90%
- Vertical membrane reduces concentration polarization for ultra-fast spin times (as fast as 10–15 minutes)
- Heat-sealed membrane minimizes downstream extractables
- 100% integrity tested for reliable performance
- Convenient sample monitoring with translucent housing and volume gradations
- Direct pipettor sample access eliminates processing step to recover concentrate
- High concentration factors of 80–100X
Applications
- Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms
- Purification of macromolecular components found in tissue culture extracts or cell lysates and protein removal prior to HPLS
- Concentration of dilute or pre-purified proteins from column eluents
- Desalting, buffer exchange and protein dialysis
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Typical Spin Times to Filtrate Volume
Spin conditions: 4000 xg swinging bucket rotor at 25°C, 15 mL sample.
3K, 10K: Cytochrome c (0.25 mg/mL); 30K, 50K: BSA (1 mg/mL); 100K: IgG (1 mg/mL).
Typical Protein Recovery
| Membrane NMWL, kDa |
Protein Solute | Protein Retentate Volume, mL |
Protein Retentate Recovery, % |
| 3 | Cytochrome c, 12.4 kDa (0.25 mg/mL) |
0.294 | 97.5 |
| 10 | Cytochrome c, 12.4 kDa (0.25 mg/mL) |
0.24 | 93.03 |
| 30 | BSA, 67 kDa (1 mg/mL) |
0.30 | 97.94 |
| 50 | BSA, 67 kDa (1 mg/mL) |
0.17 | 93.32 |
| 100 | IgG, 156 kDa (1 mg/mL) |
0.28 | 89.03 |
| 15 mL starting volume in swinging bucket rotor at 4000 x g at 25 °C. Spin times: 3K (40 min); 10K and 100K (20 min); 30K (10 min); 50K (15 min). | |||