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14-606M ASK1 Protein, active, 250 µg

Active, N-Terminal GST & C-terminal His6-tagged, recombinant, human ASK1 amino acids 649-946, for use in Kinase Assays.

ASK1 Protein, active, 250 µg MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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14-606M
250 µg  Also available in 10 μg size (cat#14-606) and in bulk (cat# 14-606-K).
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      Overview

      Replacement Information
      Description
      Catalogue Number14-606M
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionASK1 Protein, active, 250 µg
      OverviewN-Terminal GST and C-terminal His6-tagged, recombinant, human ASK1 amino acids 649-946
      References
      Product Information
      Quality LevelMQ100
      Applications
      ApplicationActive, N-Terminal GST & C-terminal His6-tagged, recombinant, human ASK1 amino acids 649-946, for use in Kinase Assays.
      Key Applications
      • Kinase Assay
      Biological Information
      Sourceexpressed in E. coli cells
      Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
      Entrez Gene Number
      Entrez Gene SummaryMitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MAPKKK5 contains 1,374 amino acids with all 11 kinase subdomains. Northern blot analysis shows that MAPKKK5 transcript is abundantly expressed in human heart and pancreas. The MAPKKK5 protein phosphorylates and activates MKK4 (aliases SERK1, MAPKK4) in vitro, and activates c-Jun N-terminal kinase (JNK)/stress-activated protein kinase (SAPK) during transient expression in COS and 293 cells; MAPKKK5 does not activate MAPK/ERK.
      Gene Symbol
      • MAP3K5
      • MEKK5
      • ASK1
      • ASK-1
      • MAPKKK5
      Protein TargetAsk1
      Purification MethodNi2+/NTA-agarose
      Target Sub-FamilySTE
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q99683 # Component of a protein kinase signal transduction cascade. Phosphorylates and activates MAP2K4 and MAP2K6, which in turn activate the JNK and p38 MAP kinases, respectively. Overexpression induces apoptotic cell death.
      COFACTOR: Magnesium.
      SIZE: 1374 amino acids; 154537 Da
      SUBUNIT: Homodimer when inactive. Binds both upstream activators and downstream substrates in multimolecular complexes. Associates with and inhibited by HIV-1 Nef. Interacts with DAB2IP.
      TISSUE SPECIFICITY: Abundantly expressed in heart and pancreas.
      SIMILARITY: SwissProt: Q99683 ## Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily. & Contains 1 protein kinase domain.
      Molecular Weight61.5kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by phosphorylation of MBP
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions6 months at -70°C
      Packaging Information
      Material Size250 µg
      Material PackageAlso available in 10 μg size (cat#14-606) and in bulk (cat# 14-606-K).
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      14-606M 04053252277733

      Related Products & Applications

      Alternative Packsize

      Catalogue Number Description  
      14-606 ASK1 Protein, active, 10 µg Show Pricing & Availability

      Product Families

      Kinase & Phosphatase Products

      Millipore offers a large portfolio of Kinases, Phophatases, Substrates, Inhibitors, Kits, reagents & tools for Signaling research. See below for a list of these products, in multiple pack sizes.
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      Categories

      Life Science Research > Proteins and Enzymes > Purified Kinases
      Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases