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GF348 TWEAK,
Human Recombinant Animal Free

TWEAK,
Human Recombinant Animal Free MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
UniProt Number: O43508
GF348
25µg  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

SourcePurity
Protein is expressed in E.coli.
The protein is manufactured without the use of any animal products/reagents and is considered Animal-Free and can be used in protocols where possible transmisson of animal viruses and contamination must be avoided.		Product endotoxin level is <0.1 ng/μg of protein (<1 EU/μg).
Description
Catalogue NumberGF348
DescriptionTWEAK,
Human Recombinant Animal Free
Alternate Names
  • TNFSF12
  • MGC129581
  • DR3LG
  • TWEAK
  • APO3L
Background InformationTWEAK belongs to the TNF family of ligands and signals through TWEAKR also known as TNFRSF12A. TWEAK is expressed in a variety of tissues, including the adult heart, pancreas, skeletal muscle, small intestine, spleen and peripheral blood lymphocytes. TWEAK has the ability to induce NFkB activation and chemokine secretion, and to exert an apoptotic activity in certain cells, such as HT-29 human adenocarcinoma cells when cultured in the presence of IFN-. TWEAK also promotes proliferation and migration of endothelial cells.
Recombinant human TWEAK is a soluble 17.0 kDa polypeptide (154 amino acid residues) comprising the TNF homologous region of TWEAK and is generated by proteolytic processing of the full length membrane anchored TWEAK protein.
References
Product Information
PresentationProduct is presented in 10 mM Sodium Phosphate, pH 7.5 + 80
mM L-Arginine and is filtered through a 0.2 micron filter before lyophilization.
Quality LevelMQ100
Applications
Biological Information
PurityProduct endotoxin level is <0.1 ng/μg of protein (<1 EU/μg).
SourceProtein is expressed in E.coli.
The protein is manufactured without the use of any animal products/reagents and is considered Animal-Free and can be used in protocols where possible transmisson of animal viruses and contamination must be avoided.
UniProt Number
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceAssay #1: The ED50 as determined by the dose-dependent stimulation of IL-8 production by human PBMC is less than 10 ng/ml.
Assay #2: TWEAK weakly induces the death of HT29 cells when cultured in the presence of IFN-γ. The ED50 for this effect is between 30-45 ng/ml.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore at -20°C for up to 4 months from date of receipt Centrifuge the vial prior to opening.
Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein and store in working aliquots at -20°C.
Packaging Information
Material Size25µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
GF348 04053252979071

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