Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

14-314 Syk Protein, active, 10 µg

Active, recombinant full-length human Syk, containing an N-terminal His6-tag, for use in Kinase Assays.

Syk Protein, active, 10 µg MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
14-314
10 µg  Also available in 250μg size -- call for pricing and availability and reference catalog number 14-314M when ordering the 250μg size.
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue Number14-314
Brand Family Upstate
Trade Name
  • Upstate
DescriptionSyk Protein, active, 10 µg
OverviewRecombinant full-length human Syk, containing an N-terminal His6-tag
References
Product Information
Quality LevelMQ100
Applications
ApplicationActive, recombinant full-length human Syk, containing an N-terminal His6-tag, for use in Kinase Assays.
Key Applications
  • Kinase Assay
Biological Information
SourceExpressed by baculovirus in Sf21 insect cells
Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Entrez Gene Number
Gene Symbol
  • SYK
Protein TargetSyk
Purification MethodNi2+/NTA-agarose
Target Sub-FamilyTK
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P43405 # Positive effector of BCR-stimulated responses. Couples the B-cell antigen receptor (BCR) to the mobilization of calcium ion either through a phosphoinositide 3-kinase-dependent pathway, when not phosphorylated on tyrosines of the linker region, or through a phospholipase C-gamma-dependent pathway, when phosphorylated on Tyr-348 and Tyr-352. Thus the differential phosphorylation of Syk can determine the pathway by which BCR is coupled to the regulation of intracellular calcium ion (By similarity).
SIZE: 635 amino acids; 72066 Da
SUBUNIT: Interacts with CBL and SLA when it is phosphorylated. The interaction with SLA may link it to CBL, leading to its destruction. Interacts with phosphorylated NFAM1 (By similarity). Interacts with Epstein-Barr virus LMP2A. Interacts through its SH2 domains with the phosphorylated ITAM domain of CD79A which stimulates SYK autophosphorylation and activation.
PTM: Autophosphorylated. & Phosphorylation on Tyr-323 creates a binding site for c-Cbl, an adapter protein that serves as a negative regulator of BCR- stimulated calcium ion signaling (By similarity). & Phosphorylation on Tyr-348 and Tyr-352 enhances the phosphorylation and activation of phospholipase C-gamma and the early phase of calcium ion mobilization via a phosphoinositide 3- kinase-independent pathway (By similarity). & Ubiquitinated by CBLB after BCR activation; which promotes proteasomal degradation (By similarity).
SIMILARITY: SwissProt: P43405 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. SYK/ZAP-70 subfamily. & Contains 1 protein kinase domain. & Contains 2 SH2 domains.
Molecular Weight73kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assuranceroutinely evaluated by phosphorylation of Poly (Glu4-Tyr)
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions1 year at -70C
Packaging Information
Material Size10 µg
Material PackageAlso available in 250μg size -- call for pricing and availability and reference catalog number 14-314M when ordering the 250μg size.
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
14-314 04053252745041

Documentation

Syk Protein, active, 10 µg SDS

Title

Safety Data Sheet (SDS) 

Syk Protein, active, 10 µg Certificates of Analysis

TitleLot Number
Syk, active - D13MP006NA D13MP006NA
Syk, active - D13NP001NA D13NP001NA
Syk, active - D8EN026U-F D8EN026U-F
Syk, active - D8EN026U-H D8EN026U-H
Syk, active - D9BN045U-B D9BN045U-B
Syk, active - D9BN045U-C D9BN045U-C

FAQ

QuestionAnswer
What is the Molar extinction coefficient for Adenosine 5'-[g32P]triphosphate ([g32P]ATP) used in the kinase assay?The Molar extinction coefficient is 15400.
What is the best way to store and thaw enzymes?In order to maintain activity of enzymes, we recommend a slow thaw and aliquot storage. To do this, remove the full vial from the freezer and place under cold running tap water. Once thawed, place the vial on ice, gently mix, than make aliquots. Using liquid nitrogen or an ethanol/dry ice bath, flash freeze the aliquots. Store aliquots at the recommended temperature.

Related Products & Applications

Alternative Packsize

Catalogue Number Description
14-314M Syk Protein, active, 250 µg

Product Families

Kinase & Phosphatase Products

Millipore offers a large portfolio of Kinases, Phophatases, Substrates, Inhibitors, Kits, reagents & tools for Signaling research. See below for a list of these products, in multiple pack sizes.
Learn More >>

Categories

Life Science Research > Proteins and Enzymes > Purified Kinases
Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases