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AC112C Sheep Anti-Human IgG Antibody, Cy3 conjugate

This Sheep Anti-Human IgG, Cy3 conjugate, Cat. No. AC112C, detects human IgG and is validated for Immunocytochemistry staining of human IgG-producing B-cell hybridoma SA13.

Sheep Anti-Human IgG Antibody, Cy3 conjugate MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Secondary & Control Antibodies
AC112C
100 µL  
Purchase on Sigma-Aldrich

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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HICCShCy3Polyclonal Antibody
Description
Catalogue NumberAC112C
DescriptionSheep Anti-Human IgG Antibody, Cy3 conjugate
Background InformationImmunoglobulin G (IgG) is part of the family of glycoprotein immunoglobulins, which stage immune responses against pathogenic organisms. IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. IgG contributes to the immune response by mobilizing innate immune effector cells and inducing and pro-inflammatory responses. Such proinflammatory responses are mediated by Fc gamma receptors which interact with sialylated Fc fragments on IgG molecules. The coupling of Cy3 to Anti-Human IgG antibody allows for the visualization of proteins by fluorescent microscopy.
References
Product Information
FormatCy3
PresentationPurified sheep polyclonal antibody Cy3 conjugate in PBS with 1.5% BSA and 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Sheep Anti-Human IgG, Cy3 conjugate, Cat. No. AC112C, detects human IgG and is validated for Immunocytochemistry staining of human IgG-producing B-cell hybridoma SA13.
Key Applications
  • Immunocytochemistry
Application NotesThis antibody is also available in unconjugated and Cy5-conjugated forms (Cat. Nos. AC112 and AC112S).
Biological Information
ImmunogenPurified human IgG.
ConcentrationPlease refer to lot specific datasheet.
HostSheep
SpecificityBased on ELISA analysis using the unconjugated antibody (Cat. No. AC112), this antibody exhibits strongest immunoreactivity towards whole human IgG molecule (IgG>IgM>IgG Fc>IgG Fab>IgA>IgM Fc) with minimal or no cross-reactivity toward IgG from chicken, guinea pig, hamster, mouse, rabbit, and rat species.
Species Reactivity
  • Human
Antibody TypePolyclonal Antibody
Purification MethodAmmonium sulfate.
Molecular Weight~150 kDa observed.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunocytochemistry in human B-cell hybridoma SA13.

Immunocytochemistry Analysis: A 1:250 dilution of this antibody immunostained 4% paraformaldehyde-fixed, 0.1% Triton X-100-permeabilized human B-cell hybridoma SA13, but not HeLa cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
AC112C 04054839114588