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555553 Rho Kinase Inhibitor III, Rockout - CAS 7272-84-6 - Calbiochem

The Rho Kinase Inhibitor III, Rockout, also referenced under CAS 7272-84-6, controls the biological activity of Rho Kinase. This small molecule/inhibitor is primarily used for Phosphorylation & Dephosphorylation applications.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: 3-(4-Pyridyl)-1H-indole

Primary Target: ROCK
555553
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Overview

Replacement Information

Key Spec Table

CAS #Empirical Formula
7272-84-6C₁₃H₁₀N₂

Products

Catalogue NumberPackaging Qty/Pack
555553-10MG Plastic ampoule 10 mg
Description
OverviewA cell-permeable indolopyridine compound that acts as a selective, reversible, and ATP-competitive inhibitor of Rho kinase activity with an IC50 of 25 µM. Does not inhibit the activation of Rho kinase nor does it affect the in vitro activities of MLCK, PKCα, and SAPK2a/p38α. Shown to be 5-fold less potent than Y-27632 (Cat. No. 688000; IC50 = ~5 µM), and display a similar specificity profile as H-89 (Cat. No. 371963). Affects cell migration, inhibits blebbing (IC50 = ~ 12 µM in M2 cells), and decreases stress fibers in Bulb 3T3 cells at 50 µM.
Catalogue Number555553
Brand Family Calbiochem®
Synonyms3-(4-Pyridyl)-1H-indole
References
ReferencesYarrow, J.C., et al. 2005. Chem. Biol. 12, 385.
Product Information
CAS number7272-84-6
ATP CompetitiveY
FormYellow solid
Hill FormulaC₁₃H₁₀N₂
Chemical formulaC₁₃H₁₀N₂
ReversibleY
Structure formula ImageStructure formula Image
Quality LevelMQ100
Applications
Biological Information
Primary TargetROCK
Primary Target IC<sub>50</sub>25 µM against ROCK
Purity≥98% by HPLC
Physicochemical Information
Cell permeableY
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage +2°C to +8°C
Protect from Light Protect from light
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Packaging Information
Packaged under inert gas Packaged under inert gas
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
555553-10MG 04055977268430

Documentation

Rho Kinase Inhibitor III, Rockout - CAS 7272-84-6 - Calbiochem SDS

Title

Safety Data Sheet (SDS) 

Rho Kinase Inhibitor III, Rockout - CAS 7272-84-6 - Calbiochem Certificates of Analysis

TitleLot Number
555553

References

Reference overview
Yarrow, J.C., et al. 2005. Chem. Biol. 12, 385.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision13-January-2010 RFH
Synonyms3-(4-Pyridyl)-1H-indole
DescriptionA cell-permeable, selective, reversible, and ATP-competitive inhibitor of Rho kinase activity (IC50 = 25 µM). Does not inhibit the activation of Rho kinase nor does it affect the in vitro activities of MLCK, PKCα, and SAPK2a/p38α. Shown to be 5-fold less potent than Y-27632 (Cat. No. 688000; IC50 ~5 µM), and display a similar specificity profile as H-89 (Cat. No. 371963). Affects cell migration, inhibits blebbing (IC50 ~12 µM in M2 cells), and decreases stress fibers in Bulb 3T3 cells at 50 µM.
FormYellow solid
Intert gas (Yes/No) Packaged under inert gas
CAS number7272-84-6
Chemical formulaC₁₃H₁₀N₂
Structure formulaStructure formula
Purity≥98% by HPLC
SolubilityDMSO (25 mg/ml) or methanol
Storage Protect from light
+2°C to +8°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Toxicity Standard Handling
ReferencesYarrow, J.C., et al. 2005. Chem. Biol. 12, 385.