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IP05 Protein G Plus/Protein A Agarose Suspension

Protein G PLUS/Protein A-Agarose mixture specifically formulated for immunoprecipitation.

Protein G Plus/Protein A Agarose Suspension MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
IP05
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Overview

Products

Catalogue NumberPackaging Qty/Pack
US1IP05-1.5ML Plastic ampoule 1.5 ml
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Description
OverviewDesigned for immunoprecipitation applications. This product is blocked with BSA to reduce non-specific binding and cannot be used for purification.
Catalogue NumberIP05
Brand Family Calbiochem®
Product Information
FormLiquid slurry
Formulation33% slurry in PBS.
Preservative≤0.1% sodium azide
Applications
Key Applications Immunoprecipitation
Application NotesImmunoprecipitation (see comments)
Application CommentsAgarose solution is supplied ready to use. Protein G Plus/Protein A Agarose immunoprecipitation reagent is blocked with BSA and should not be used for immunoglobulin purification or covalent cross-linking. For immunoprecipitation reactions 15 µl of solution per µg primary antibody is recommended. Preclearing will minimize extra bands resulting from nonspecific precipitation. To preclear, add to the sample 20 µl of agarose conjugate and 1 µg of normal IgG from the same species as the immunoprecipitating antibody. When immunoblotting is used for detection, some secondary antibodies can react nonspecifically with BSA or other proteins present at high concentrations in the sample. This can be eliminated by reducing the concentration of secondary antibody.
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Yes
Global Trade Item Number
Catalogue Number GTIN
US1IP05-1.5ML 04055977220223