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IP02 MilliporeProtein A Agarose Suspension

Protein A Agarose Suspension designed for immunoprecipitation applications

Protein A Agarose Suspension MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

SDS
CoA
Data Sheet

Recommended Products

Overview

Replacement Information

Products

Catalogue Number	Packaging	Qty/Pack
US1IP02-1.5ML	Plastic ampoule	1.5 ml	Add To Favorites Add To Favorites Added To My Favorites

Description
Overview	Designed for immunoprecipitation applications. This product is blocked with BSA to reduce non-specific binding and cannot be used for purification; best for mouse IgG_2a and IgG_2b and rabbit IgG.
Catalogue Number	IP02
Brand Family	Calbiochem®

References

Product Information
Form	Liquid slurry
Formulation	In PBS.
Preservative	≤0.1% sodium azide

Applications
Key Applications	Immunoprecipitation
Application Comments	Agarose solutions are supplied ready-to-use for immunoprecipitation. Use 15 µl suspension/µg primary antibody. Protein A-Agarose is recommended for use in immunoprecipitation assays using rabbit polyclonal antibodies or unmodified mouse IgG_2a or IgG_2b monoclonal antibodies. Mouse IgG₁ or IgG₃ and all rat antibodies bind poorly to Protein A. As an alternative, use Protein G PLUS, Protein G PLUS/Protein A, or rabbit second-step antibodies to bridge these antibodies to Protein A. Preclearing will minimize extra bands resulting from nonspecific precipitation. To preclear, add to the sample 20 µl of agarose conjugate and 1 µg of normal IgG from the same species as the immunoprecipitating antibody. When immunoblotting is used for detection, some secondary antibodies can react nonspecifically with BSA or other proteins present at high concentrations in the sample. This can be eliminated by reducing the concentration of secondary antibody.

Applications

Key Applications

Immunoprecipitation

Application Comments

Agarose solutions are supplied ready-to-use for immunoprecipitation. Use 15 µl suspension/µg primary antibody. Protein A-Agarose is recommended for use in immunoprecipitation assays using rabbit polyclonal antibodies or unmodified mouse IgG_2a or IgG_2b monoclonal antibodies. Mouse IgG₁ or IgG₃ and all rat antibodies bind poorly to Protein A. As an alternative, use Protein G PLUS, Protein G PLUS/Protein A, or rabbit second-step antibodies to bridge these antibodies to Protein A. Preclearing will minimize extra bands resulting from nonspecific precipitation. To preclear, add to the sample 20 µl of agarose conjugate and 1 µg of normal IgG from the same species as the immunoprecipitating antibody. When immunoblotting is used for detection, some secondary antibodies can react nonspecifically with BSA or other proteins present at high concentrations in the sample. This can be eliminated by reducing the concentration of secondary antibody.

Biological Information

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements

Storage and Shipping Information
Ship Code	Blue Ice Only
Toxicity	Standard Handling
Storage	+2°C to +8°C
Do not freeze	Yes

Packaging Information

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
Catalogue Number	GTIN
US1IP02-1.5ML	04055977220209

Documentation

Protein A Agarose Suspension SDS

Title
Safety Data Sheet (SDS)

Protein A Agarose Suspension Certificates of Analysis

Title	Lot Number
IP02	Enter Lot Number

Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision	04-September-2007 RFH
Description	Protein A conjugated to agarose.
Form	Liquid slurry
Formulation	In PBS.
Preservative	≤0.1% sodium azide
Comments	Agarose solutions are supplied ready-to-use for immunoprecipitation. Use 15 µl suspension/µg primary antibody. Protein A-Agarose is recommended for use in immunoprecipitation assays using rabbit polyclonal antibodies or unmodified mouse IgG_2a or IgG_2b monoclonal antibodies. Mouse IgG₁ or IgG₃ and all rat antibodies bind poorly to Protein A. As an alternative, use Protein G PLUS, Protein G PLUS/Protein A, or rabbit second-step antibodies to bridge these antibodies to Protein A. Preclearing will minimize extra bands resulting from nonspecific precipitation. To preclear, add to the sample 20 µl of agarose conjugate and 1 µg of normal IgG from the same species as the immunoprecipitating antibody. When immunoblotting is used for detection, some secondary antibodies can react nonspecifically with BSA or other proteins present at high concentrations in the sample. This can be eliminated by reducing the concentration of secondary antibody.
Storage	+2°C to +8°C
Do Not Freeze	Yes
Toxicity	Standard Handling

Related Products

Catalogue Number	Description
IP04	Protein G Plus-Agarose Suspension
IP05	Protein G Plus/Protein A Agarose Suspension
IP08	Protein G Plus-Agarose
IP10	Protein G Plus/Protein A-Agarose

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