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525295 PhosphoDetect™ Anti-Phosphotyrosine Mouse mAb (PY20)

This PhosphoDetect™ Anti-Phosphotyrosine Mouse mAb (PY20) is validated for use in ELISA, Immunoblotting, Frozen Sections, ICC, IP for the detection of Phosphotyrosine.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
525295
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
A Broad Range Of SpeciesMMonoclonal Antibody

Products

Catalogue NumberPackaging Qty/Pack
525295-100UG Plastic ampoule 100 μg
Description
OverviewRecognizes a broad spectrum of tyrosine-phosphorylated proteins in their native and denatured states in crude extracts.
Catalogue Number525295
Brand Family Calbiochem®
References
ReferencesGlenney, J.R., et al. 1988. J. Immunol. Methods 109, 277.
Product Information
FormLiquid
FormulationIn 10 mM sodium phosphate, pH 7.4.
Positive controlAny cell lysate or tissue extract
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Application ReferencesImmunoblotting Dixon, B.S., et al. 1996. J. Biol. Chem. 271, 13324. Frozen Sections, Immunocytochemistry Bao, Z.Z., et al. 1993. J. Cell Sci. 106, 579.
Key Applications Enzyme-Linked Immunosorbent Assay
Frozen Sections
Immunoblotting (Western Blotting)
Immunocytochemistry
Immunoprecipitation
Application NotesELISA (see comments)
Immunoblotting (1:500-1:1000, see application references)
Frozen Sections (see application references)
Immunocytochemistry (see application references)
Immunoprecipitation (see comments)
Application CommentsBinding is inhibited by phosphotyrosine. Does not react with threonine or serine phosphorylated residues on proteins. Do not use milk as a blocking agent with this antibody. Milk contains many phosphotyrosines and will adsorb the antibody. This antibody has also been reported to work for ELISA and immunoprecipitation. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
ImmunogenKLH conjugated to phosphotyrosine, glycine, and alanine
ClonePY20
HostMouse
IsotypeIgG2b
Species Reactivity
  • A Broad Range Of Species
Antibody TypeMonoclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
525295-100UG 04055977270686

Documentation

PhosphoDetect™ Anti-Phosphotyrosine Mouse mAb (PY20) SDS

Title

Safety Data Sheet (SDS) 

PhosphoDetect™ Anti-Phosphotyrosine Mouse mAb (PY20) Certificates of Analysis

TitleLot Number
525295

References

Reference overview
Glenney, J.R., et al. 1988. J. Immunol. Methods 109, 277.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision03-December-2007 JSW
ApplicationELISA (see comments)
Immunoblotting (1:500-1:1000, see application references)
Frozen Sections (see application references)
Immunocytochemistry (see application references)
Immunoprecipitation (see comments)
DescriptionPurified mouse monoclonal antibody. Recognizes a broad spectrum of tyrosine-phosphorylated proteins in their native and denatured states.
BackgroundCellular activation, proliferation and differentiation are signaled by phosphorylation of tyrosine residues. Growth factor receptors and oncogene protein kinases are associated with tyrosine phosphorylation. Anti-phosphotyrosine antibodies are indispensable for the study of cellular tyrosine phosphorylation.
HostMouse
ImmunogenKLH conjugated to phosphotyrosine, glycine, and alanine
ClonePY20
IsotypeIgG2b
Speciesa broad range of species
Positive controlAny cell lysate or tissue extract
FormLiquid
FormulationIn 10 mM sodium phosphate, pH 7.4.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsBinding is inhibited by phosphotyrosine. Does not react with threonine or serine phosphorylated residues on proteins. Do not use milk as a blocking agent with this antibody. Milk contains many phosphotyrosines and will adsorb the antibody. This antibody has also been reported to work for ELISA and immunoprecipitation. Variables associated with assay conditions will dictate the proper working dilution.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesGlenney, J.R., et al. 1988. J. Immunol. Methods 109, 277.
Application referencesImmunoblotting Dixon, B.S., et al. 1996. J. Biol. Chem. 271, 13324. Frozen Sections, Immunocytochemistry Bao, Z.Z., et al. 1993. J. Cell Sci. 106, 579.