Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

516726 Phenol/Chloroform/Isoamyl Alcohol, 25:24:1 (v/v), Molecular Biology Grade - Calbiochem

A 25:24:1 (v/v) mixture of phenol, chloroform and isoamyl alcohol, pH 6.7. Suitable for DNA and RNA applications.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
516726
Purchase on Sigma-Aldrich

Overview

Replacement Information

Products

Catalogue NumberPackaging Qty/Pack
516726-1SET Metal can 1 set
Description
OverviewA 25:24:1 (v/v) mixture of phenol, chloroform and isoamyl alcohol, pH 6.7. Suitable for DNA and RNA applications. Absorbance: ≤0.2 at 300 nm; and ≤0.1 at 405 nm and 510 nm. Volume 400 ml. Supplied with 26 ml Tris-based equilibration buffer.
Catalogue Number516726
Brand Family Calbiochem®
References
Product Information
FormTwo-layer colorless liquid
Quality LevelMQ100
Applications
Biological Information
Purity≥99% by titration (phenol); >99% by HPLC (chloroform)
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
R PhraseR: 23/24/25-34-40

Toxic by inhalation, in contact with skin and if swallowed.
Causes burns.
Limited evidence of a carcinogenic effect.
S PhraseS: 26-27-36/37/39-45

In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
Take off immediately all contaminated clothing.
Wear suitable protective clothing, gloves and eye/face protection.
In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Toxic
Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
Storage +2°C to +8°C
Protect from Light Protect from light
Do not freeze Ok to freeze
Special InstructionsFollowing equilibration, refrigerate (4°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
516726-1SET 07790788050993

Documentation

Phenol/Chloroform/Isoamyl Alcohol, 25:24:1 (v/v), Molecular Biology Grade - Calbiochem SDS

Title

Safety Data Sheet (SDS) 

Phenol/Chloroform/Isoamyl Alcohol, 25:24:1 (v/v), Molecular Biology Grade - Calbiochem Certificates of Analysis

TitleLot Number
516726
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision27-January-2010 RFH
DescriptionA 25:24:1 (v/v) mixture of phenol, chloroform and isoamyl alcohol, pH 6.7. Suitable for DNA and RNA applications. Prior to equilibration with the Tris-based equilibration buffer (pH ~6.7), the mix is ideal for extraction of proteins and lipids for RNA purification. Following equilibration with the Tris-based equilibration buffer (pH ~8.0), is ideal for extraction of proteins and lipids for DNA purification. Absorbance: ≤0.2 at 300 nm; and ≤0.1 at 405 nm and 510 nm. Volume 400 ml. Supplied with 26 ml Tris-based equilibration buffer.
FormTwo-layer colorless liquid
Recommended reaction conditions
Equilibration To equilibrate add the entire bottle (26 ml) of Equilibration Buffer, mix and allow to stand for 1 h before use.
Purity≥99% by titration (phenol); >99% by HPLC (chloroform)
Storage Protect from light
+2°C to +8°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing equilibration, refrigerate (4°C).
Toxicity Toxic