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109269 Papanicolaou's solution 3d polychromatic solution EA 65

for cytology

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
109269
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Overview

Products

Catalogue NumberPackaging Qty/Pack
1092690100 Glass bottle 100 ml
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1092692500 Glass bottle 2.5 l
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Description
Catalogue Number109269
DescriptionPapanicolaou’s solution 3d polychromatic solution EA 65
OverviewPapanicolaou’s solution 3d polychromatic solution EA 65 - for cytology, is a ready-to-use solution used for human-medical cell diagnosis and serve the purpose of the cytological investigation of sample material of human origin, for example, gynecological and clinico-cytological specimen materials. Papanicolaou’s technique is the most used staining procedure for cytological specimen is and is intended for the the staining of exfoliative cells in cytological specimens. In the first step, the cell nuclei are stained either progressively or regressively with a hematoxylin solution, (Hematoxylin solution modified acc.to Gill II, (Cat.no. 105175), Papanicolaou’s solution 1a Harris hematoxylin solution, (Cat.no. 109253) or Papanicolaou’s solution 1b Hematoxylin solution S, (Cat.no. 109254)). Nuclei are stained blue to dark violet. In the progressive hematoxylin staining method, staining is carried out to the endpoint, after which the slide is blued in tapwater. With the regressive method the material is over-stained and the excess of staining solution is removed by acid rinsing steps, followed by the bluing step. The structures of nuclei are more differentiated and better visible by the regressive method. The second staining step is cytoplasmic staining by orange staining solution (Papanicolaou’s solution 2b Orange II solution, Cat. no. 1106887 or Papanicolaou’s solution 2a Orange G solution (OG6), Cat. no. 106888), especially for demonstration of mature and keratinized cells. The target structures are stained orange in different intensities. In the third staining step is used the so-called polychrome solution, a mixture of eosin, light green SF and Bismarck brown, (Papanicolaou’s solution 3a polychromatic solution EA 31, (Cat.no 109271), Papanicolaou’s solution 3b polychromatic solution EA 50, (Cat.no. 109272), Papanicolaou’s solution 3c polychromatic solution EA 65,(Cat.no. 109270), or Papanicolaou’s solution 3d polychromatic solution EA 65, (Cat.no.109269). The polychrome solution is used for demonstration of differentiation of squamous cells. Papanicolaou’s solution 3d polychromatic solution EA 65 gives a delicate, blue-green-pinkish staining result in clinical specimen material.
The 100 ml bottle provides 300 - 400 stainings. It is registered as IVD and CE certified product and can be used for clinical diagnostics. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.
Product Information
HS Code3212 90 00
Quality LevelMQ400
Applications
Applicationfor cytology
Physicochemical Information
Density0.85 g/cm3 (20 °C)
Flash point17 °C
Safety Information according to GHS
Hazard Pictogram(s) Hazard Pictogram(s)
Hazard Pictogram(s)
Hazard Statement(s)H225: Highly flammable liquid and vapour.
H317: May cause an allergic skin reaction.
H319: Causes serious eye irritation.
Precautionary Statement(s)P210: Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
P233: Keep container tightly closed.
P240: Ground and bond container and receiving equipment.
P280: Wear protective gloves/ protective clothing/ eye protection/ face protection/ hearing protection.
P303 + P361 + P353: IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water.
P305 + P351 + P338: IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
P403 + P233: Store in a well-ventilated place. Keep container tightly closed.
Signal WordDanger
Storage class3 Flammable liquids
WGKWGK 2 obviously hazardous to water
Disposal1
Strongly contaminated halogen-free organic solvents: container A.
Safety Information
Categories of dangerhighly flammable
Storage and Shipping Information
StorageStore at +15°C to +25°C.
Transport Information
Declaration (railroad and road) ADR, RIDUN 1170 , 3, II
Declaration (transport by air) IATA-DGRUN 1170 , 3, II
Declaration (transport by sea) IMDG-CodeUN 1170 , 3, II
Specifications
Suitability for microscopy (Vaginal smear)passes test
Nucleiblue to dark violet
Cyanophilic Cytoplasm (basophilic)blue-green to blue-grey
Eosinophilic Cytoplasm (acidophilic)pink to red-violet
Global Trade Item Number
Catalogue Number GTIN
1092690100 04022536112787
1092692500 04022536112800