Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

14-505M PKC iota Recombinant Human Protein, Active, 250 µg

Active, N-terminal His6-tagged, recombinant full-length human PKC iota. For use in Kinase Assays.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
14-505M
250 µg  Also available in 10 μg size (cat#14-505) and in bulk (cat# 14-505-K).
Purchase on Sigma-Aldrich

Overview

Description
Catalogue Number14-505M
Brand Family Upstate
Trade Name
  • Upstate
DescriptionPKC iota Recombinant Human Protein, Active, 250 µg
OverviewN-terminal His6-tagged, recombinant full-length human PKC iota

PKC Lipid activator (Catalog # 20-133) is required for use.
Product Information
Quality LevelMQ100
Applications
ApplicationActive, N-terminal His6-tagged, recombinant full-length human PKC iota. For use in Kinase Assays.
Key Applications
  • Kinase Assay
Biological Information
SourceHuman PKC iota expressed in Sf21 cells
Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Entrez Gene Number
Entrez Gene SummaryThis gene encodes a member of the protein kinase C (PKC) family of serine/threonine protein kinases. The PKC family comprises at least eight members, which are differentially expressed and are involved in a wide variety of cellular processes. This protein kinase is calcium-independent and phospholipid-dependent. It is not activated by phorbolesters or diacylglycerol. This kinase can be recruited to vesicle tubular clusters (VTCs) by direct interaction with the small GTPase RAB2, where this kinase phosphorylates glyceraldehyde-3-phosphate dehydrogenase (GAPD/GAPDH) and plays a role in microtubule dynamics in the early secretory pathway. This kinase is found to be necessary for BCL-ABL-mediated resistance to drug-induced apoptosis and therefore protects leukemia cells against drug-induced apoptosis. There is a single exon pseudogene mapped on chromosome X.
Gene Symbol
  • PRKCI
  • DXS1179E
  • MGC26534
  • PRKC-lambda/iota
  • PKCI
  • aPKC-lambda/iota
  • nPKC-iota
Protein TargetPKCι
Purification MethodNi2+/NTA-agarose
Target Sub-FamilyAGC
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P41743 # Calcium-independent, phospholipid-dependent, serine- and threonine-specific kinase. May play a role in the secretory response to nutrients. Involved in cell polarization processes and the formation of epithelial tight junctions. Implicated in the activation of several signaling pathways including Ras, c-Src and NFkappa-B pathways. Functions in both pro- and anti-apoptotic pathways. Functions in the RAC1/ERK signaling required for transformed growth. Plays a role in microtubule dynamics through interaction with RAB2 and GAPDH and recruitment to vesicular tubular clusters (VTCs).
SIZE: 587 amino acids; 67258 Da
SUBUNIT: Forms a complex with SQSTM1 and MP2K5 (By similarity). Interacts directly with SQSTM1 (Probable). Interacts with IKBKB. Interacts with PARD6A, PARD6B and PARD6G. Part of a quaternary complex containing aPKC, PARD3, a PARD6 protein (PARD6A, PARD6B or PARD6G) and a GTPase protein (CDC42 or RAC1). Part of a complex with LLGL1 and PARD6B. Interacts with CENTA1. Interaction with SMG1, through the ZN-finger domain, activates the kinase activity. Interacts with CDK7. Forms a complex with RAB2 and GAPDH involved in recruitment onto the membrane of vesicular tubular clusters (VTCs).
SUBCELLULAR LOCATION: Cytoplasm. Membrane. Endosome. Nucleus. Note=Transported into the endosome through interaction with SQSTM1/p62. After phosphorylation by cSrc, transported into the nucleus through interaction with KPNB1. Colocalizes with CDK7 in the cytoplasm and nucleus. Vesicular tubular clusters. Transported to VTCs through interaction with Rab2.
TISSUE SPECIFICITY: Predominantly expressed in lung and brain, but also expressed at lower levels in many tissues including pancreatic islets. Highly expressed in non-small cell lung cancers.
DOMAIN: SwissProt: P41743 The OPR domain mediates interaction with SQSTM1 (By similarity). & The C1 domain does not bind diacylglycerol (DAG).
PTM: On neuronal growth factor (NGF) stimulation, phosphorylated by Src on Tyr-256, Tyr-271 and Tyr-325. Phosphorylation on Tyr-256 facilitates binding to KPNB1/importin-beta regulating entry of PRKCI into the nucleus. Phosphorylation on Tyr-325 is important for NF-kappaB stimulation.
SIMILARITY: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily. & Contains 1 AGC-kinase C-terminal domain. & Contains 1 OPR domain. & Contains 1 phorbol-ester/DAG-type zinc finger. & Contains 1 protein kinase domain.
Molecular Weight68.6kDa
Product Usage Statements
Quality AssuranceRoutinely evaluated by phosphorylation of PKC Substrate Peptide
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions6 months at -70°C
Packaging Information
Material Size250 µg
Material PackageAlso available in 10 μg size (cat#14-505) and in bulk (cat# 14-505-K).
Global Trade Item Number
Catalogue Number GTIN
14-505M 04053252402593