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14-676 NLK Protein, active, 10 µg

Active, N-terminal 6His-tagged, recombinant, human NLK, amino acids 121-end, for use in Kinase Assays.

NLK Protein, active, 10 µg MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
14-676
10 µg  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue Number14-676
Brand Family Upstate
Trade Name
  • Upstate
DescriptionNLK Protein, active, 10 µg
OverviewN-terminal 6His-tagged, recombinant, human NLK, amino acids 121-end
References
Product Information
Quality LevelMQ100
Applications
ApplicationActive, N-terminal 6His-tagged, recombinant, human NLK, amino acids 121-end, for use in Kinase Assays.
Key Applications
  • Kinase Assay
Biological Information
Sourceexpressed by baculovirus in Sf21 cells
Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Entrez Gene Number
Gene Symbol
  • NLK
  • DKFZp761G1211
  • FLJ21033
  • LAK1
Protein TargetNLK
Purification MethodNi2+/NTA-agarose
Target Sub-FamilyCMGC
UniProt Number
UniProt SummaryFUNCTION: SwissProt: Q9UBE8 # Role in cell fate determination, required for differentiation of bone marrow stromal cells. Acts downstream of MAP3K7 and HIPK2 to negatively regulate the canonical Wnt/beta- catenin signaling pathway and the phosphorylation and destruction of the MYB transcription factor. May suppress a wide range of transcription factors by phosphorylation of the coactivator, CREBBP (By similarity).
COFACTOR: Magnesium (By similarity).
SIZE: 515 amino acids; 57048 Da
SUBUNIT: Interacts with HIPK2 and MYB.
SUBCELLULAR LOCATION: Nucleus (By similarity). Cytoplasm (By similarity). Note=Predominantly nuclear. A smaller fraction is cytoplasmic (By similarity).
PTM: Dually phosphorylated on Thr-291 and Tyr-293, which activates the enzyme (By similarity).
SIMILARITY: SwissProt: Q9UBE8 ## Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily. & Contains 1 protein kinase domain.
Molecular Weight48.7kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assuranceroutinely evaluated by phosphorylation of myelin basic protein (Catalog # 13-104)
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 6 months at -70°C from date of shipment
Packaging Information
Material Size10 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
14-676 04053252580635