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411550 Mouse Anti-Human IgG, Fc Fragment Specific (HP6043) Peroxidase Conjugate

This Mouse Anti-Human IgG, Fc Fragment Specific (HP6043) Peroxidase Conjugate is validated for use in Enzyme Immunoassay for the detection of Human IgG, Fc Fragment Specific.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
411550
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Overview

Products

Catalogue NumberPackaging Qty/Pack
411550-500UG Glass bottle 500 μg
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Description
Catalogue Number411550
Brand Family Calbiochem®
References
ReferencesHamilton, R.G. 1993. Clin. Chem. 39, 1988.
Hamilton, R.G. and Morrison, S.M. 1993. J. Immunolo. Meth. 158, 107.
Hamilton, R.G. 1990. Ann. Biol. Clin. 48, 473.
Hamilton, R.G., et al. 1988. J. Immunoassay. 9, 275.
Hamilton, R.G., et al. 1987. Hybridoma 6, 204.
Nakane, P.K. and Kawaoi A., 1974. J. Histochem Cytochem. 22, 1084.
Product Information
FormLiquid
FormulationIn PBS, 10 mg/ml BSA.
PreservativeNone
Quality LevelMQ200
Applications
Application ReferencesEnzyme Immunoassay Hamilton, R.G., et al. 1987. Hybridoma 6, 204. Cross-Reactivity, Myeloma Proteins Hamilton, R.G., et al. 1988. J. Immunoassay. 9, 275. Hamilton, R.G., et al. 1987. Hybridoma 6, 204.
Key Applications Enzyme Immunoassay
Application NotesEnzyme Immunoassay (1:1000, see application references)
Application CommentsAntibody should be titrated for optimal results in individual systems.

Cross-reactivity by ELISA against human myeloma proteins:
Human IgG1: 100%
Human IgG2: 100%
Human IgG3: 100%
Human IgG4: 100%
Human IgM: <0.2%
Human IgA: <0.1%
Human IgE: <0.1%

Cross-reactivity by ELISA against chimeric antibodies:
Human IgG1: 100%
Human IgG2: 100%
Human IgG3: 100%
Human IgG4: 100%
Human IgM: <0.01%
Human IgA: <0.01%
Human IgE: <0.01%

There is no detectable binding to bovine, goat, horse sheep IgG, so these species sera can be used for assay blocking buffers. Modification of Nakane and Kawaoi method used for enzyme coupling.
Biological Information
CloneHP6043
HostMouse
IsotypeIgG
Antibody TypeMonoclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C). Avoid more than 5 freeze/thaw cycles.
Global Trade Item Number
Catalogue Number GTIN
411550-500UG 07790788060275