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FCMAB323PE Milli-Mark™ Anti-Phosphotyrosine-PE Antibody, recombinant clone 4G10®

FCMAB323PE
100 tests  
Purchase on Sigma-Aldrich

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Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
VrtFCMPhycoerythrinMonoclonal Antibody
Description
Catalogue NumberFCMAB323PE
Trade Name
  • Milli-Mark
DescriptionMilli-Mark™ Anti-Phosphotyrosine-PE Antibody, recombinant clone 4G10®
Background InformationSome of the tyrosine residues can be tagged with a phosphate group by protein kinases and in its phosphorylated state, it is referred to as phosphotyrosine. Tyrosine phosphorylation is considered as one of the key steps in signal transduction and regulation of enzymatic activity.
The advent of anti-phosphotyrosine antibodies is one of the significant events in signal transduction research. Before the availability of anti-phosphotyrosine antibodies, tyrosine phosphorylation of proteins and enzymes was investigated through hazardous and time-consuming radioactive experiments. Anti-phosphotyrosine antibodies are commonly used in western blots after the targeted proteins have been immunoprecipitated to measure the tyrosyne phosphorylation of the proteins. Anti-phosphotyrosine antibodies are also directly used on cell lysate to examine the overall change of tyrosine phosphorylation level in reponse to various treatments.
References
Product Information
FormatPhycoerythrin
Control
  • Treated versus untreated A431 cells
PresentationPurified mouse monoclonal IgG2bk conjugated to PE in PBS with 0.1% sodium azide and 15 mg/mL BSA.
Quality LevelMQ100
Applications
ApplicationMilli-Mark Anti-Phosphotyrosine-PE Antibody, recombinant clone 4G10 is an antibody against Phosphotyrosine clone 4G10 for use in FC.
Key Applications
  • Flow Cytometry
Biological Information
ImmunogenPhosphotyramine-KLH
Clone4G10
HostMouse
SpecificityAntibody recognizes tyrosine-phosphorylated proteins from all species
IsotypeIgG2bκ
Species Reactivity
  • Vertebrates
Species Reactivity NoteTested and passed on Human. Expected to detect tyrosyne phosphorylated proteins in all species.
Antibody TypeMonoclonal Antibody
Purification MethodProtein G Purified
Molecular WeightDependent upon the molecular weight of the tyrosine phosphorylated protein being detected.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by flow cytometry using EGF treated versus untreated A431 cells
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain refrigerated at 2-8°C protected from light for up to 6 months from date of receipt.
Packaging Information
Material Size100 tests
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
FCMAB323PE 04053252749537

Documentation

Milli-Mark™ Anti-Phosphotyrosine-PE Antibody, recombinant clone 4G10® SDS

Title

Safety Data Sheet (SDS) 

Milli-Mark™ Anti-Phosphotyrosine-PE Antibody, recombinant clone 4G10® Certificates of Analysis

TitleLot Number
Milli-Mark Anti-Phosphotyrosine-PE, recombinant clone 4G10 - NG1897024 NG1897024
Milli-Mark Anti-Phosphotyrosine-PE, recombinant clone 4G10#174; - 1990437 1990437
Milli-Mark Anti-Phosphotyrosine-PE, recombinant clone 4G10#174; - 2024543 2024543
Milli-Mark Anti-Phosphotyrosine-PE, recombinant clone 4G10#174; - 2042800 2042800
Milli-Mark Anti-Phosphotyrosine-PE, recombinant clone 4G10#174; - 2069371 2069371
Milli-Mark Anti-Phosphotyrosine-PE, recombinant clone 4G10#174; - 2110524 2110524
Milli-Mark Anti-Phosphotyrosine-PE, recombinant clone 4G10#174; - 2201508 2201508
Milli-Mark™ Anti-Phosphotyrosine-PE, -2569807 2569807
Milli-Mark™ Anti-Phosphotyrosine-PE, -2677919 2677919
Milli-Mark™ Anti-Phosphotyrosine-PE, recombinant clone 4G10 2487766