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14-643M MRCKβ Protein, active, 250 µg

Active, N-terminal His6-tagged, recombinant, human MRCKb amino acids 1-473, for use in Kinase Assays.

14-643M
250 µg  Also available in 10 μg size (cat#14-643) and in bulk (cat#14-643-K).
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue Number14-643M
Brand Family Upstate
Trade Name
  • Upstate
DescriptionMRCKβ Protein, active, 250 µg
OverviewN-terminal His6-tagged, recombinant, human MRCKb amino acids 1-473
References
Product Information
Quality LevelMQ300
Applications
ApplicationActive, N-terminal His6-tagged, recombinant, human MRCKb amino acids 1-473, for use in Kinase Assays.
Key Applications
  • Kinase Assay
Biological Information
Sourcehuman MRCKb amino acids 1-473, expressed by baculovirus in Sf21 cells
Entrez Gene Number
Entrez Gene SummaryThis gene encodes a member of the serine/threonine protein kinase family. The encoded protein contains a Cdc42/Rac-binding p21 binding domain resembling that of PAK kinase. The kinase domain of this protein is most closely related to that of myotonic dystrophy kinase-related ROK. Studies of the similar gene in rat suggested that this kinase may act as a downstream effector of Cdc42 in cytoskeletal reorganization.
Gene Symbol
  • CDC42BPB
  • KIAA1124
  • MRCKB
Protein TargetMRCKβ
Purification MethodNi2+/NTA-agarose
Target Sub-FamilyAGC
UniProt Number
UniProt SummaryFUNCTION: SwissProt: Q9Y5S2 # May act as a downstream effector of CDC42 in cytoskeletal reorganization. Contributes to the actomyosin contractility required for cell invasion, through the regulation of MYPT1 and thus MLC2 phosphorylation (By similarity).
COFACTOR: Magnesium (By similarity).
SIZE: 1711 amino acids; 194315 Da
SUBUNIT: Homodimer and homotetramer via the coiled coil regions. Interacts tightly with GTP-bound but not GDP-bound CDC42 (By similarity).
SUBCELLULAR LOCATION: Cytoplasm (By similarity). Note=Displays a dispersed punctate distribution and concentrates along the cell periphery, especially at the leading edge and cell-cell junction. This concentration is PH-domain dependent (By similarity).
TISSUE SPECIFICITY: Expressed in all tissues examined, with high levels in heart, brain, placenta and lung.
SIMILARITY: SwissProt: Q9Y5S2 ## Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. DMPK subfamily. & Contains 1 AGC-kinase C-terminal domain. & Contains 1 CNH domain. & Contains 1 CRIB domain. & Contains 1 PH domain. & Contains 1 phorbol-ester/DAG-type zinc finger. & Contains 1 protein kinase domain.
Molecular Weight55.5kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceRoutinely evaluated by phosphorylation of S6 Kinase/Rsk2 Substrate Peptide 2
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions6 months at -70°C
Packaging Information
Material Size250 µg
Material PackageAlso available in 10 μg size (cat#14-643) and in bulk (cat#14-643-K).
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
14-643M 04053252924514

Documentation

Related Products & Applications

Alternative Packsize

Catalogue Number Description
14-643 MRCKβ Protein, active, 10 µg

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Categories

Life Science Research > Proteins and Enzymes > Purified Kinases
Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases