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48-682MAG MILLIPLEX MAP Bcl-2 Family Apoptosis Panel 1 Magnetic Bead 6-plex Kit - Cell Signaling Multiplex Assay

Intracellular Bead-Based Multiplex Assays using the Luminex technology enable the simultaneous relative quantitation of multiple phosphorylation and total pathway proteins in tissue and cell lysate samples. Compare Multiplexing results to those of Western blotting.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Multiplexing Detection Methods: Luminex xMAP Research Category: Cell Signaling
48-682MAG
96-well plate  96-well plate
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
BAD (Ser112) BAD (Total) BIM BAX Bcl-xL/BAD Mcl-1/BIM HMplexLuminex xMAP
Description
Catalogue Number48-682MAG
Trade Name
  • MILLIPLEX
DescriptionMILLIPLEX MAP Bcl-2 Family Apoptosis Panel 1 Magnetic Bead 6-plex Kit - Cell Signaling Multiplex Assay
OverviewApoptosis is an essential physiological process that plays a crucial role in normal development and homeostatic mechanisms of multicellular organisms. At least two major apoptotic pathways have been described in mammalian cells: the intrinsic pathway via Caspase-9 activation, in which death arises from mitochondrial dysfunction, and the extrinsic pathway via Caspase-8 activation, in which death is initiated from the activation of cell surface receptors. Bcl-2 family members play an important role in the intrinsic apoptotic pathway and includes over 17 members. Interactions of these Bcl-2 family members function as a ‘life/death switch’ that integrates diverse inter- and intracellular cues to determine whether or not the stress apoptosis pathway should be activated. For example, Bcl-2 and its closely related anti-apoptotic proteins, Bcl-xL and Mcl-1, can sequester their pro-apoptotic counterparts (BAD, BIM, NOXA and PUMA) and thereby inhibit the initiation steps of apoptosis..

The MILLIPLEX® MAP 6-plex Bcl-2 Family Apoptosis Panel 1 Magnetic Bead Kit (Cat. No. 48-682MAG) is a premixed kit to be used for the simultaneous quantification of the following 6 analytes:

• pBAD (Ser112)
• BAD (total)
• BIM (total)
• BAX (total)
• Bcl-xL/BAD
• Mcl-1/BIM
References
Product Information
Detection methodLuminex xMAP
ConfigurationPremixed
Precision, %
  • Intra-Assay: < 15
  • Inter-Assay: < 20
Panel TypeCell Signaling
Quality LevelMQ100
Applications
ApplicationIntracellular Bead-Based Multiplex Assays using the Luminex technology enable the simultaneous relative quantitation of multiple phosphorylation and total pathway proteins in tissue and cell lysate samples. Compare Multiplexing results to those of Western blotting.
Key Applications
  • Multiplexing
Application Notes• An overnight (4°C) incubation is recommended for best results.
• This assay requires 25 µL diluted cell lysate per well.
• This kit must be run using Assay Buffer 1 (provided).
• The suggested working range of protein concentration for the assay is 1 to 25 µg of total protein/well (25 μL/well at 40 to 1,000 µg/mL).
Biological Information
SpecificityCross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
Species Reactivity
  • Human
Analytes Available
  • BAD (Ser112)
  • BAD (Total)
  • BIM
  • BAX
  • Bcl-xL/BAD
  • Mcl-1/BIM
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage for kit components is 2 - 8°C.
Packaging Information
Material Size96-well plate
Material Package96-well plate
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
48-682MAG 04054839444272