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HCYTPAB-96SK MILLIPLEX® Human Cytokine Panel A + B 96-Plex Combo Pack

Simultaneously analyze 48 cytokine, chemokine, and growth factor biomarkers with each Bead-Based Multiplex Assay using the Luminex technology, in human serum, plasma and cell culture samples.

HCYTPAB-96SK
96-well plate  2 kits to be run separately
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberHCYTPAB-96SK
Trade Name
  • MILLIPLEX
DescriptionMILLIPLEX® Human Cytokine Panel A + B 96-Plex Combo Pack
OverviewMILLIPLEX® offers the broadest selection of analytes across a wide range of disease states and species. Once the analytes of interest have been identified, you can rely on the quality that we build into each kit to produce results you can trust. In addition to the assay characteristics listed in the protocol, other performance criteria evaluated during the verification process include: cross-reactivity, dilution linearity, kit stability, and sample behavior (e.g. detectability and stability).

Each MILLIPLEX® panel and kit includes:
• Quality controls (QCs) to qualify assay performance
• Comparison of standard (calibrator) and QC lots to a reference lot to ensure lot-to-lot consistency
• Optimized serum matrix to mimic native analyte environment in serum and plasma samples
• Detection antibody cocktails designed to yield consistent analyte profiles within panel

In addition each panel and kit meets stringent manufacturing criteria to ensure batch-to-batch reproducibility. The MILLIPLEX® Human Cytokine/Chemokine/Growth Factor Panel A thus enables you to focus on the therapeutic potential of cytokines and the modulation of cytokine expression. Coupled with the Luminex® xMAP® platform in a magnetic bead format, you receive the advantage of ideal speed and sensitivity, allowing quantitative multiplex detection of dozens of analytes simultaneously, which can dramatically improve productivity.
Background InformationCytokines, chemokines, and growth factors are key mediators of immune system functions capable of signaling through autocrine, paracrine, and endocrine mechanisms. Their pleiotropic immunomodulatory properties allow these biomolecules to react to diverse stimuli and regulate the immune response, either by promoting or inhibiting inflammation. Growing research continues to highlight the role of the immune system in every facet of human health and disease.
References
Product Information
Detection methodLuminex xMAP
ConfigurationPremixed
Precision, %
  • Intra-Assay: <15%
  • Inter-assay: <20%
Panel TypeMAGNETIC Cytokines/Chemokines
Applications
ApplicationSimultaneously analyze 48 cytokine, chemokine, and growth factor biomarkers with each Bead-Based Multiplex Assay using the Luminex technology, in human serum, plasma and cell culture samples.
Key Applications
  • Multiplexing
Application Notes• This is an overnight or two-hour incubation assay.
• Each assay requires 25 µL of neat plasma or serum, or 25 μL cell culture supernatant per well.
• Two kits are included and are intended to be run separately.
Biological Information
Species Reactivity
  • Human
Analytes Available
  • EGF
  • Eotaxin
  • G-CSF
  • GM-CSF
  • IFNα2
  • IFNγ
  • IL-1α
  • IL-1β
  • IL-1ra
  • IL-2
  • IL-3
  • IL-4
  • IL-5
  • IL-6
  • IL-7
  • IL-8
  • IL-12 (p40)
  • IL-12(p70)
  • IL-13
  • IL-15
  • IL-17A
  • IL-17E/IL-25
  • IL-17F
  • IL-18
  • IL-22
  • IP-10/CXCL10
  • MCP-1
  • M-CSF
  • MIG
  • MIP-1α
  • MIP-1β
  • PDGF-AA
  • PDGF-AB/BB
  • RANTES
  • TNFα
  • TNFβ
  • VEGF-A
  • 6Ckine
  • sCD137
  • sFas
  • sFasL
  • APRIL/TNFSF13
  • BAFF/Blys
  • BCA-1
  • CTACK
  • ENA-78
  • Eotaxin-2
  • Eotaxin-3
  • Granzyme A
  • Granzyme B
  • HMGB1
  • I-309
  • IFNβ
  • IL-16
  • IL-20
  • IL-21
  • IL-23
  • IL-28A
  • IL-29
  • IL-31
  • IL-33
  • I-TAC
  • LIF
  • MCP-2
  • MCP-4
  • MIP-1d
  • MIP-3α
  • Perforin
  • SCF
  • SDF-1
  • TARC
  • TPO
  • TRAIL
  • TSLP
  • sCD40L
  • FGF-2
  • Flt-3
  • Fractalkine
  • MDC (CCL22)
  • IL-11
  • IL-24
  • IL-34
  • MIP-3β
  • MPIF-1/CCL23
  • GROα
  • IL-9
  • IL-10
  • MCP-3
  • TGFα
  • CCL28
  • CXCL16
  • IL-35
  • GCP2
  • IFNω
  • Lymphotactin
Cross ReactivityThere was no or negligible cross-reactivity observed between the antibodies for any single analyte and any of the other analytes in this panel, except IL-12 (p40). IL-12 (p40) exhibited a cross-reactivity of approximately 8% with IL-12 (p70). This is not unexpected since the p40 subunit is a constituent of IL-12 (p70).
Accuracy
  • 81%-106%
Physicochemical Information
Sensitivity
  • Refer to kit protocol for sensitivities for individual cytokines, chemokines and growth factors.
Standard Curve Range
  • MIP-1β: 0.38 - 6,000 pg/mL
  • IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-18, MDC: 0.64 - 10,000 pg/mL
  • FLT-3L: 0.96 - 15,000 pg/mL
  • GROα, IFNγ, IL-3, IL-17A, RANTES, TGFα: 1.3 - 20,000 pg/mL
  • IL-1β, IL-1RA, TNFβ: 1.6 - 25,000 pg/mL
  • GM-CSF, IL-10, IP-10, VEGF-A: 2.6 - 40,000 pg/mL
  • EGF, Eotaxin, IL-12 (p70), IL-15, MCP-1, MIP-1α: 3 - 50,000 pg/mL
  • G-CSF, IL-1α: 4.8 - 75,000 pg/mL
  • IL-12 (p40), IL-13, MIG, TNFα: 6.4 - 100,000 pg/mL
  • IFNα2, MCP-3: 8 - 125,000 pg/mL
  • PDGF-AB/BB: 9.6 - 150,000 pg/mL
  • sCD40L, IL-22, PDGF-AA: 13 - 200,000 pg/mL
  • IL-27: 16 - 250,000 pg/mL
  • FGF-2: 26 - 400,000 pg/mL
  • Fractalkine, IL-17F: 32 - 500,000 pg/mL
  • IL-17E/IL-25, M-CSF: 40 - 625,000 pg/mL
  • 6CKine: 37-150,000 pg/mL
  • BCA-1, MCP-4: 1.7-7,000 pg/mL
  • sFas: 0.3-1,100 ng/mL
  • sFasL, IL-33: 20-80,000 pg/mL
  • CTACK: 3.7-15,000 pg/mL
  • CXCL16, Eotaxin-3, IL-21, IL-28A: 4.9-20,000 pg/mL
  • ENA-78: 3.1-12,500 pg/mL
  • GCP-2: 0.9-3,500 pg/mL
  • sCD137, APRIL, Granzyme A, Eotaxin-2, IFNβ, IFNω, IL-11, IL-31, LIF, TRAIL: 2.4-10,000 pg/mL
  • BAFF: 13-55,000 pg/mL
  • IL-16, IL-34, MIP-1δ: 10-40,000 pg/mL
  • CCL28: 61-250,000 pg/mL
  • IL-35, SCF, SDF-1, TPO: 12-50,000 pg/mL
  • HMGB1: 134-550,000 pg/mL
  • I-309: 1.5-6,000 pg/mL
  • Granzyme B: 1.8-7,500 pg/mL
  • IL-23, IL-24: 24-100,000 pg/mL
  • IL-20: 15-60,000 pg/mL
  • IL-29: 6.1-25,000 pg/mL
  • Lymphotactin: 7.3-30,000 pg/mL
  • MCP-2: 0.2-1,000 pg/mL
  • MIP-3α: 0.6-2,500 pg/mL
  • I-TAC, MIP-3β: 1.2-5,000 pg/mL
  • MPIF-1: 1.0-4,000 pg/mL
  • TARC: 0.8-3,250 pg/mL
  • TSLP: 0.5-2,000 pg/mL
  • Perforin: 18-75,000 pg/mL
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage for kit components is 2 - 8°C.
Packaging Information
Material Size96-well plate
Material Package2 kits to be run separately
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
HCYTPAB-96SK 04065270020079

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