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14-429M MEK1 Protein, active, 250 µg

Active, N-terminal GST & C-terminal His6-tagged, recombinant full-length human MEK1, for use in Kinase Assays.

MEK1 Protein, active, 250 µg MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
14-429M
250 µg  Also available in 10 μg size (cat#14-429) and in bulk (cat# 14-429-K).
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue Number14-429M
Brand Family Upstate
Trade Name
  • Upstate
DescriptionMEK1 Protein, active, 250 µg
OverviewN-terminal GST and Cterminal His6-tagged, recombinant full-length human MEK1
References
Product Information
Quality LevelMQ100
Applications
ApplicationActive, N-terminal GST & C-terminal His6-tagged, recombinant full-length human MEK1, for use in Kinase Assays.
Key Applications
  • Kinase Assay
Biological Information
Sourceexpressed in E. coli
Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Entrez Gene Number
Entrez Gene SummaryThe protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development.
Gene Symbol
  • MAP2K1
  • MAPKK1
  • PRKMK1
  • MEK1
  • MKK1
Protein TargetMEK1
Purification MethodPurified using glutathione agarose followed by Ni2+/NTA agarose, activated using c-Raf and the re-purified using Ni2+/NTA agarose
Target Sub-FamilySTE
UniProt Number
UniProt SummaryFUNCTION: SwissProt: Q02750 # Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates ERK1 and ERK2 MAP kinases.
SIZE: 393 amino acids; 43439 Da
SUBUNIT: Interacts with MORG1 (By similarity). Interacts with Yersinia yopJ.
PTM: Phosphorylation on Ser/Thr by MAP kinase kinase kinases (RAF or MEKK1) regulates positively the kinase activity. & Acetylation by Yersinia yopJ prevents phosphorylation and activation, thus blocking the MAPK signaling pathway.
DISEASE: SwissProt: Q02750 # Defects in MAP2K1 are a cause of cardiofaciocutaneous syndrome (CFC syndrome) [MIM:115150]; also known as cardio-facio- cutaneous syndrome. CFC syndrome is characterized by a distinctive facial appearance, heart defects and mental retardation. Heart defects include pulmonic stenosis, atrial septal defects and hypertrophic cardiomyopathy. Some affected individuals present with ectodermal abnormalities such as sparse, friable hair, hyperkeratotic skin lesions and a generalized ichthyosis-like condition. Typical facial features are similar to Noonan syndrome. They include high forehead with bitemporal constriction, hypoplastic supraorbital ridges, downslanting palpebral fissures, a depressed nasal bridge, and posteriorly angulated ears with prominent helices. The inheritance of CFC syndrome is autosomal dominant.
SIMILARITY: SwissProt: Q02750 ## Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily. & Contains 1 protein kinase domain.
Molecular Weight71kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assuranceroutinely evaluated by phosphorylation of MBP
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions1 year at -70°C
Packaging Information
Material Size250 µg
Material PackageAlso available in 10 μg size (cat#14-429) and in bulk (cat# 14-429-K).
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
14-429M 04053252336669

Related Products & Applications

Alternative Packsize

Catalogue Number Description
14-429 MEK1 Protein, active, 10 µg

Product Families

Kinase & Phosphatase Products

Millipore offers a large portfolio of Kinases, Phophatases, Substrates, Inhibitors, Kits, reagents & tools for Signaling research. See below for a list of these products, in multiple pack sizes.
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Categories

Life Science Research > Proteins and Enzymes > Purified Kinases
Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases