Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

SCT011 LuminiCell Tracker™ 670- Cell Labeling Kit

Biocompatible organic fluorescent nanoparticles (AIEDots) that can be used to label cells for long term cell tracking and cell tracing in vitro and in vivo experiments.

SCT011
1 Kit  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberSCT011
DescriptionLuminiCell Tracker™ 670- Cell Labeling Kit
OverviewAbsorbance Maximum: 510nm
Emisson Maximum: 670nm

Suggested Filters:
Excitation: 458/488/543nm
Emisson: 670-800nm
Alternate Names
  • AIEDots Cell Tracker Dye
  • AIE Live Cell Fluorescent Dye
  • Fluorescent Cell Tracking Dye
  • Live Cell Fluorescent Nanoparticles
Background InformationLong-term noninvasive cell tracking by fluorescent probes and quantum dots is of great importance to life science and biomedical engineering. Current methods used to fluorescently tag cells have been limited by short signal duration, high background auto-fluorescence or lengthy molecular cloning manipulations using GFP. LuminiCell Trackers™ are biocompatible organic fluorescent nanoparticles based on Aggregation Induced Emission technology, termed AIEDots. Aggregation induced emission (AIE) molecules emit fluorescence in an opposite manner than other common fluorophores (Quantum Dots, GFP). Propeller-shaped AIE fluorogens are non-emissive in solutions but become highly fluorescent upon aggregate formation. Due to these differences, LuminiCell Trackers™ have very high fluorescence intensities with minimal signal quenching allowing live cell fluorescent tagging for up to 10 days in vitro and 21 days in vivo. These properties make them optimal candidates for long interval live cell bioimaging experiments.

The Luminicell Tracker™-670 Cell Labeling Kit contain red fluorescent AIEDot nanoparticles with TAT sequences. These nanoparticles can be used to fluorescently tag cells for long term cell tracking and tracing experiments.

Features and Benefits

● Brighter: 10X brighter than other cell labeling technologies
● Photostable: 3X longer fluorescence without signal quenching
● Biocompatible: Non-toxic organic nanoparticles intended for biological applications
● Rapid Protocol: Easy-to-use protocol labels cells within 4 hours

References

1) Liu B, Tang BZ et al. Photostable fluorescent organic dots with aggregation-induced emission (AIE dots) for noninvasive long-term cell tracing. Sci Rep. 2013;3:1150.
2) Kang Y et al. Long-Term Tracking Mesenchymal Stem Cell Differentiation with Photostable Fluorescent Nanoparticles. ACS Appl Mater Interfaces. 2016 May 18;8(19):11925-33.
3) Tang, B. Aggregation-Induced Emission: Together We Shine, United We Soar! Chem Rev. 2015 Nov 11;115(21):11718-940.
References
Product Information
HS Code3204 20 90
Presentation20nM in 0.5ml PBS, pH 7.4
Quality LevelMQ100
Applications
ApplicationBiocompatible organic fluorescent nanoparticles (AIEDots) that can be used to label cells for long term cell tracking and cell tracing in vitro and in vivo experiments.
Key Applications
  • Cell Culture
  • Fluorescence
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceAbsorbance: 510+/-5nm
Concentration: 180-220nM
Fluorescence: 665+/- 10nm
Brightness at 670nm: ≥1.7X10^7 M-1cm-1
Cellular Assay: HeLa Cell Fluorescence
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore at -2-8°C upon receipt. Thaw at room temperature or in a water bath. Do not Freeze.

Note: Some particulates may form as a result of nanoparticle aggregation during shipping. To get particulates back in solution, sonicate the vial containing LuminiCell Tracker™ three times for 1 min each before use.
Packaging Information
Material Size1 Kit
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
SCT011 04054839235368

Related Products & Applications