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427211 Lactate Dehydrogenase, Porcine Heart

427211
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Overview

Replacement Information

Products

Catalogue NumberPackaging Qty/Pack
427211-50KU Plastic ampoule 50 ku
Description
OverviewNative lactate dehydrogenase from porcine heart. A tetrameric enzyme that catalyzes the reversible conversion of pyruvate to lactate. The overall equilibrium of this reaction favors lactate formation. Note: 1KU = 1000 units.
Note: 1 KU = 1000 units
Catalogue Number427211
Brand Family Calbiochem®
References
ReferencesKornberg, A. 1995. Methods Enzymol. 1, 441
Product Information
CAS number9001-60-9
Unit of DefinitionOne unit is defined as the amount of enzyme that will oxidize 1.0 µmol NADH per min at 25°C, pH 7.4.
EC number1.1.1.27
FormLyophilized
PI4.6
Quality LevelMQ100
Applications
Biological Information
Specific Activity≥300 units/mg protein. One unit is defined as the amount of enzyme that will cause the oxidation of one micromole of NADH per minute at 25°C, pH 7.4
Physicochemical Information
ContaminantsGPT, GOT: ≤0.03%; PK, MK: ≤0.01%; MDH: ≤0.1%
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage -20°C
Protect from Moisture Protect from moisture
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, refrigerate (4°C). Stock solutions are stable for up to 24 h at 4°C. Do not freeze solutions.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
427211-50KU 04055977187397

Documentation

Lactate Dehydrogenase, Porcine Heart SDS

Title

Safety Data Sheet (SDS) 

Lactate Dehydrogenase, Porcine Heart Certificates of Analysis

TitleLot Number
427211

References

Reference overview
Kornberg, A. 1995. Methods Enzymol. 1, 441
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision24-August-2009 JSW
DescriptionNative lactate dehydrogenase from porcine heart. A tetrameric enzyme that catalyzes the reversible conversion of pyruvate to lactate. The overall equilibrium of this reaction favors lactate formation. Has an optimal pH of 7.0 and a pI of 4.6. Note: 1KU = 1000 units.
FormLyophilized
CAS number9001-60-9
EC number1.1.1.27
ContaminantsGPT, GOT: ≤0.03%; PK, MK: ≤0.01%; MDH: ≤0.1%
Specific activity≥300 units/mg protein. One unit is defined as the amount of enzyme that will cause the oxidation of one micromole of NADH per minute at 25°C, pH 7.4
Unit definitionOne unit is defined as the amount of enzyme that will oxidize 1.0 µmol NADH per min at 25°C, pH 7.4.
SolubilityAqueous Buffers
Storage Protect from moisture
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, refrigerate (4°C). Stock solutions are stable for up to 24 h at 4°C. Do not freeze solutions.
Toxicity Standard Handling
Merck USA index14, 5334
ReferencesKornberg, A. 1995. Methods Enzymol. 1, 441