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14-328 JNK1α1/SAPK1c Protein, inactive, 50 µg

Inactive, N-terminal His6-tagged recombinant, human, full-length JNK1α1/SAPK1c, for use in Kinase Assays.

JNK1α1/SAPK1c Protein, inactive, 50 µg MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
14-328
50 µg  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue Number14-328
Brand Family Upstate
Trade Name
  • Upstate
DescriptionJNK1α1/SAPK1c Protein, inactive, 50 µg
OverviewN-terminal His6-tagged recombinant, human, full-length JNK1α1/SAPK1c
References
Product Information
Quality LevelMQ100
Applications
ApplicationInactive, N-terminal His6-tagged recombinant, human, full-length JNK1α1/SAPK1c, for use in Kinase Assays.
Key Applications
  • Kinase Assay
Biological Information
SourceExpressed by baculovirus in Sf21 insect cells
Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Entrez Gene Number
Entrez Gene SummaryThe protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrom c-mediated cell death pathway. Studies of the mouse counterpart of this gene suggested that this kinase play a key role in T cell proliferation, apoptosis and differentiation. Four alternatively spliced transcript variants encoding distinct isoforms have been reported.
Gene Symbol
  • MAPK8
  • JNK1
  • JNK21B1/2
  • PRKM8
  • JNK-46
  • JNK
  • SAPK1
  • JNK1A2
Protein TargetJNK1α1/SAPK1c
Purification MethodNi2+/NTA-agarose
Target Sub-FamilyCMGC
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P45983 # JNK1 isoforms display different binding patterns: beta-1 preferentially binds to c-Jun, whereas alpha-1, alpha-2, and beta- 2 have a similar low level of binding to both c-Jun or ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms.
COFACTOR: Magnesium.
SIZE: 427 amino acids; 48296 Da
SUBUNIT: Binds to at least four scaffolding proteins, MAPK8IP1/JIP-1, MAPK8IP2/JIP-2, MAPK8IP3/JIP-3/JSAP1 and SPAG9/MAPK8IP4/JIP-4. These proteins also bind other components of the JNK signaling pathway. Interacts with TP53 and WWOX. Interacts with JAMP (By similarity).
DOMAIN: SwissProt: P45983 The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
PTM: Dually phosphorylated on Thr-183 and Tyr-185, which activates the enzyme.
SIMILARITY: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily. & Contains 1 protein kinase domain.
Molecular Weight45kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceRoutinely evaluated by phosphorylation of ATF2 (amino acids 19-196)
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions6 months at -20°C
Packaging Information
Material Size50 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
14-328 04053252406102

Documentation

JNK1α1/SAPK1c Protein, inactive, 50 µg SDS

Title

Safety Data Sheet (SDS) 

JNK1α1/SAPK1c Protein, inactive, 50 µg Certificates of Analysis

TitleLot Number
JNK1#945;1, unactive - 1603534-E 1603534-E
JNK1#945;1, unactive - 1603534-F 1603534-F

References

Reference overviewPub Med ID
Transcription factor ATF2 regulation by the JNK signal transduction pathway.
Gupta, S, et al.
Science, 267: 389-93 (1995)  1995

Show Abstract 		7824938 7824938