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IBFP0813C Immobilon® Blotting Filter Paper

8.5 x 13.5 cm sheet, Chromotography-Grade Blotting Paper for western blotting.

Immobilon® Blotting Filter Paper MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Western Blotting, Dot Blot
IBFP0813C
100  
Purchase on Sigma-Aldrich

Overview

Key Spec Table

Key Applications
WB, DB
Description
Catalogue NumberIBFP0813C
Trade Name
  • Immobilon
DescriptionImmobilon® Blotting Filter Paper
Background InformationOverview
- Millipore offers four different Immobilon PVDF membranes, each optimized for different protein blotting applications.
Immobilon PVDF membranes provide many advantages compared to nitrocellulose. They won’t crack or curl, and they can be cut without fracturing. They also have low background, broad solvent compatibility, and superior staining capabilities. In addition, they can be reprobed multiple times.

Immobilon-P Transfer Membrane:
- 45 µm pore size Recommended for most western blotting, especially proteins greater than 20 kDa
- Millipore's Rapid Immunodetection Protocol reduces detection times by up to 2 hours by eliminating membrane blocking and several wash steps with no loss of sensitivity or specificity

Immobilon-E Membrane:
- the only PVDF membrane that wets out in aqueous buffers, eliminating the methanol pre-wet step.
- 45 µm pore size recommended for most western blotting, especially proteins greater than 20 kDa

Blotting Sandwiches:
- When you need to process multiple blots, blotting sandwiches are a convenient, time-saving choice. Our sandwiches include sheets of Immobilon-P membrane interleaved with pre-cut sheets of chromatography-grade blotting filter paper and are compatible with any standard wet transfer device.

Blotting Filter Paper:
- Chromatography-grade blotting filter paper pre-cut to the most popular western blotting sizes.

Immobilon-PSQ Membrane:
- 0.2 µm pore size and a large internal structure
- Higher protein adsorption and sequencing yields than other membranes
- Recommended for blotting proteins less than 20 kDa
- Prevents blow-through of low molecular weight proteins

Immobilon-FL Membrane:
- The first transfer membrane optimized for fluorescence applications
- Extremely low background improves sensitivity of all fluorescence detection protocols
- Compatible with all commonly used fluorescent probes at all excitation and emission wavelengths
- Ideal for multiplexing and chemifluorescence applications

Immobilon® PVDF membranes are offered in four types, each optimized for a different protein blotting application. Convenient blotting sandwiches feature pre-cut sheets of membrane and blotting filter paper. Immobilon® PVDF membranes have high protein adsorption, so you won’t lose proteins during transfer or reprobing. The open pore structure makes it easy to access bound proteins and remove unbound probes. In addition, Immobilon® PVDF membranes optimized for fluorescent blots dramatically increase signal-to-noise ratios for high sensitivity in quantitative, multiplexing applications.

Features & Benefits:
- Won’t crack, curl or fracture when cut
- Low background
- Superior staining capabilities
- Can be reprobed multiple times

Applications:
- Western Blotting, Immunoblotting, Dot Blotting, Protein Sequencing;
- Compatible with Chemiluminescent, Fluorescent, Radioactive, Chromogenic, and Chemifluorescent Detection
- Compatible with all commonly used fluorescent probes at all excitation and emission wavelengths
Product Information
Detection methodFluorescent Chemifluorescence Chromogenic Chemiluminescence
Compatible Stains
  • Ponceau-S red
  • India ink
  • Colloidal gold
  • CPTS
Filter CodeIBFP
Filter ColorWhite
Quality LevelMQ400
Applications
Application8.5 x 13.5 cm sheet, Chromotography-Grade Blotting Paper for western blotting.
Key Applications
  • Western Blotting
  • Dot Blot
Biological Information
BSA AdsorptionNot Applicable
WettabilityHydrophilic
Physicochemical Information
Pore SizeNot Applicable
Dimensions
Filter SurfacePlain
Filter Dimensions8.5 cm x 13.5 cm
Thickness550 µm
Materials Information
Chemistry
  • Chromotography-Grade Blotting Paper
Packaging Information
Material Size100
Global Trade Item Number
Catalogue Number GTIN
IBFP0813C 04053252327575