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SCC145 ID8 Mouse Ovarian Surface Epithelial Cell Line

ID8 mouse ovarian surface epithelial cell line is frequently used as a syngeneic mouse model for human ovarian cancer.

SCC145
≥1X10⁶ cells/vial  
Purchase on Sigma-Aldrich

Overview

Description
Catalogue NumberSCC145
DescriptionID8 Mouse Ovarian Surface Epithelial Cell Line
OverviewID8 is one of 10 clonal lines established from late passaged C57BL/6 murine ovarian surface epithelial cells (MOSEC) (1). Intraperitoneal injection of each of the 10 clonal lines into C57BL/6 mice resulted in formation of peritoneal tumors and ascitic fluid. Of the 10 clonal lines, ID8 exhibited the highest tumor load. ID8 cell line is a highly published and well characterized cell line and is frequently used as a syngeneic mouse model for ovarian cancer.
Alternate Names
  • ID-8
  • ID8/MOSEC
Background InformationOvarian cancer is the fourth leading cause of cancer-related deaths in women. The disease is frequently diagnosed at later stages, with tumors detected throughout the peritoneal cavity. Approximately 90% of ovarian tumors arise from ovarian surface epithelial cells (2). Human and mouse ovarian surface epithelial cells (OSE) have been isolated and are used to develop ovarian cancer models. These models typically involve injection of the human/mouse OSE cells subcutaneously, intraperitoneally or orthotopically into immune deficient mice. A common drawback to these models is the absence of an intact immune system in the host mice.

In 2000, an immune-competent syngeneic mouse model for ovarian cancer was reported (1). Mouse ovarian surface epithelial cells (MOSEC) isolated from C57BL/6 mice were found to spontaneously transform into malignant tumorigenic cells following prolonged passages in vitro (1). Late passage MOSEC lost the classical “cobblestone” contact-inhibited in vitro properties reminiscent of normal epithelial cells and instead grew as multi-layered cell clusters indicative of transformed cells. Intraperitoneal injection of late passaged MOSEC into athymic and normal, immune-intact, syngeneic C57BL/6 mice gave rise to tumors throughout the abdominal cavity like those observed in women with Stage III and IV cancer (1). MOSEC are thus useful syngeneic mouse models to study the role of the immune system in the establishment and progression of ovarian cancer.

References:
1. Carcinogenesis 2000; 21(4): 585-91.
2. Gynecol Oncol 2008; 108(2): 385-94.
Product Information
HS Code3002 59 00
Applications
ApplicationID8 mouse ovarian surface epithelial cell line is frequently used as a syngeneic mouse model for human ovarian cancer.
Key Applications
  • Cell Culture
Application NotesThis product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the “Academic Use Agreement” as detailed in the product documentation. For information regarding any other use, please contact licensing@emdmillipore.com.
Biological Information
Cell Line Type
  • Cancer Cells
Product Usage Statements
Quality Assurance• Each vial contains ≥ 1X106 viable cells.
• Cells are tested negative for infectious diseases by a Mouse Essential CLEAR panel by Charles River Animal Diagnostic Services.
• Cells are verified to be of mouse origin and negative for inter-species contamination from rat, chinese hamster, Golden Syrian hamster, human and non-human primate (NHP) as assessed by a Contamination CLEAR panel by Charles River Animal Diagnostic Services.
• Cells are negative for mycoplasma contamination
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Packaging Information
Material Size≥1X10⁶ cells/vial
Global Trade Item Number
Catalogue Number GTIN
SCC145 04054839501937