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EZHADP-61BK Human Adiponectin ELISA- Bulk

This Human Adiponectin ELISA is used to measure & quantify Adiponectin levels in Metabolism & Endocrine research.

Human Adiponectin ELISA- Bulk MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: ELISA Detection Methods: Fluorescent, Colorimetric Research Category: Metabolism Research Sub-Category: Obesity, Metabolic Disorders
EZHADP-61BK
10 Human Adiponectin ELISA kits  This product contains 10 Human Adioponectin ELISA kits.
Purchase on Sigma-Aldrich

Overview

Replacement Information
Replacement InformationThis product replaces CYT350, CHEMICON® Human Adiponectin ELISA Kit

Key Spec Table

Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
Adiponectin HELISAFluorescent Colorimetric
Description
Catalogue NumberEZHADP-61BK
ReplacesCYT350
DescriptionHuman Adiponectin ELISA- Bulk
Background InformationAdiponectin is identified as a 30 kDa adipokine that is secreted from the adipose tissue into circulating blood and influences systemic metabolism.
References
Product Information
Detection methodFluorescent Colorimetric
Precision, %
  • Inter-assay: 1.0–7.4 , 2.4–8.4
  • Intra-assay: 1.0–7.4
Quality LevelMQ200
Applications
ApplicationThis Human Adiponectin ELISA is used to measure & quantify Adiponectin levels in Metabolism & Endocrine research.
Key Applications
  • ELISA
Application NotesRoom Temperature, 3 hour assay using 20 µL sample size (serum/plasma: 1:500 dilution)
Biological Information
Species Reactivity
  • Human
Analytes Available
  • Adiponectin
Entrez Gene Number
Entrez Gene SummaryThis gene is expressed in adipose tissue exclusively. It encodes a protein with similarity to collagens X and VIII and complement factor C1q. The encoded protein circulates in the plasma and is involved with metabolic and hormonal processes.
Gene Symbol
  • ADIPOQ
  • adiponectin
  • AdipoQ
  • apM-1
  • APM1
  • ACDC
  • GBP28
  • APM-1
  • ACRP30
  • apM1
UniProt Number
UniProt SummaryFUNCTION: SwissProt: Q15848 # Important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW.
SIZE: 244 amino acids; 26414 Da
SUBUNIT: Homomultimer. Forms trimers, hexamers and 12- to 18-mers. The trimers (low molecular weight complexes / LMW) are assembled via non-covalent interactions of the collagen-like domains in a triple helix and hydrophobic interactions within the globular C1q domain. Several trimers can associate to form disulfide-linked hexamers (middle molecular weight complexes / MMW) and larger complexes (higher molecular weight / HMW). The HMW-complex assembly may rely aditionnally on lysine hydroxylation and glycosylation. LMW, MMW and HMW complexes bind to HBEGF, MMW and HMW complexes bind to PDGFB, and HMW complex binds to FGF2.
SUBCELLULAR LOCATION: Secreted.
TISSUE SPECIFICITY: Synthesized exclusively by adipocytes and secreted into plasma.DOMAIN:SwissProt: Q15848 The C1q domain is commonly called the globular domain.
PTM: Hydroxylated Lys-33 was not identified in PubMed:16497731, probably due to poor representation of the N-terminal peptide in mass fingerprinting. & HMW complexes are more extensively glycosylated than smaller oligomers. Hydroxylation and glycosylation of the lysine residues within the collagene-like domain of adiponectin seem to be critically involved in regulating the formation and/or secretion of HMW complexes and consequently contribute to the insulin- sensitizing activity of adiponectin in hepatocytes (By similarity). & O-linked glycans consist of Glc-Gal disaccharides bound to the oxygen atom of post-translationally added hydroxyl groups. & Not N-glycosylated.
DISEASE: SwissProt: Q15848 # Defects in ADIPOQ are the cause of adiponectin deficiency [MIM:605441]. The result is a very low concentration of plasma adiponectin. Decreased adiponectin plasma levels are associated with obesity insulin resistance, and diabetes type 2.
SIMILARITY: Contains 1 C1q domain. & Contains 1 collagen-like domain.
MISCELLANEOUS: Variants Arg-84 and Ser-90 show impaired formation of HMW complexes whereas variants Cys-112 and Thr-164 show impaired secretion of adiponectin in any form. & HMW-complex blood contents are higher in females than in males, are increased in males by castration and decreased again upon subsequent testosterone treatment, which blocks HMW- complex secretion (By similarity). In type 2 diabetic patients, both the ratios of HMW to total adiponectin and the degree of adiponectin glycosylation are significantly decreased as compared with healthy controls.
Accuracy
  • 92–102%
Physicochemical Information
Sensitivity
  • 1.5 ng/mL
Linearity of Dilution81–100%
Standard Curve Range
  • 1.5–100 ng/mL adiponection
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • For research use only. Not for use in diagnostic procedures.
Storage and Shipping Information
Packaging Information
Material Size10 Human Adiponectin ELISA kits
Material PackageThis product contains 10 Human Adioponectin ELISA kits.
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
EZHADP-61BK 04053252436925