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20-175 HDAC Assay Buffer, 5X

The protein encoded by this gene belongs to the class II histone deacetylase/acuc/apha family. It possesses histone deacetylase activity & represses transcription when tethered to a promoter.

HDAC Assay Buffer, 5X MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Histone Deacetylase Assay (HDAC)
20-175
10 mL  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Key Applications
HDAC
Description
Catalogue Number20-175
Brand Family Upstate
Trade Name
  • Upstate
DescriptionHDAC Assay Buffer, 5X
References
Product Information
Presentation5X HDAc Assay Buffer: 50mM Tris-HCl, pH 8.0, 750mM NaCl and 50% glycerol.
Quality LevelMQ100
Applications
ApplicationThe protein encoded by this gene belongs to the class II histone deacetylase/acuc/apha family. It possesses histone deacetylase activity & represses transcription when tethered to a promoter.
Key Applications
  • Histone Deacetylase Assay (HDAC)
Application NotesFor use in the Histone Deacetylase Assay (HDAC) Kit.
Biological Information
Entrez Gene Number
Entrez Gene SummaryHistones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to the class II histone deacetylase/acuc/apha family. It possesses histone deacetylase activity and represses transcription when tethered to a promoter. It coimmunoprecipitates only with HDAC3 family member and might form multicomplex proteins. It also interacts with myocyte enhancer factor-2 (MEF2) proteins, resulting in repression of MEF2-dependent genes. This gene is thought to be associated with colon cancer. Two transcript variants encoding different isoforms have been found for this gene.
Gene Symbol
  • HDAC1
  • RPD3L1
  • GON-10
  • DKFZp686H12203
  • HD1
  • RPD3
UniProt Number
UniProt SummaryFUNCTION: SwissProt: Q9UQL6 # Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation by repressing transcription of myocyte enhancer MEF2C. During muscle differentiation, it shuttles into the cytoplasm, allowing the expression of myocyte enhancer factors.
SIZE: 1122 amino acids; 121992 Da
SUBUNIT: Interacts with BCOR, HDAC7, HDAC9, CTBP1, MEF2C, NCOR2, NRIP1, PHB2 and a 14-3-3 chaperone protein. Interacts with JARID1B.
SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Note=Shuttles between the nucleus and the cytoplasm. In muscle cells, it shuttles into the cytoplasm during myocyte differentiation. The export to cytoplasm depends on the interaction with a 14-3-3 chaperone protein and is due to its phosphorylation at Ser-259 and Ser-498 by CaMK.TISSUE SPECIFICITY: Ubiquitous.DOMAIN:SwissProt: Q9UQL6 The nuclear export sequence mediates the shuttling between the nucleus and the cytoplasm (By similarity).
PTM: Phosphorylated by CaMK at Ser-259 and Ser-498. The phosphorylation is required for the export to the cytoplasm. & Ubiquitinated. Polyubiquitination however does not lead to its degradation.
SIMILARITY: Belongs to the histone deacetylase family. Type 2 subfamily.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions1 year at -20°C
Packaging Information
Material Size10 mL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
20-175 04053252032769

Documentation

HDAC Assay Buffer, 5X SDS

Title

Safety Data Sheet (SDS) 

HDAC Assay Buffer, 5X Certificates of Analysis

TitleLot Number
HDAC Assay Buffer, 5X 2934466
HDAC Assay Buffer, 5X - 3782151 3782151
HDAC Assay Buffer, 5X - 3866435 3866435
HDAC Assay Buffer, 5X - Any-lot Any-lot

References

Reference overviewPub Med ID
A novel protein, Mpm1, of the mitochondria of the yeast Saccharomyces cerevisiae.
H Inadome,Y Noda,H Adachi,K Yoda
Bioscience, biotechnology, and biochemistry  65  2001

Show Abstract 		11791739 11791739