Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

101603 Gram-Color modified

(phenol-free) staining kit for Gram staining method on bacteriological smears

Gram-Color modified MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
101603
Purchase on Sigma-Aldrich

Overview

Replacement Information

Products

Catalogue NumberPackaging Qty/Pack
1016030001 Fibre case 1 set
Description
Catalogue Number101603
DescriptionGram-Color modified
OverviewThe Gram-Color modified (phenol-free) - staining kit for Gram staining method on bacteriological smears, is used for human-medical cell diagnosis and serves the purpose of the bacteriological investigation of sample material of human origin from smears of body fluids. Gram-Color modified is a staining kit which is used for a modified Gram staining. The Gram-Color modified solutions are modified and designed in such a way that staining can be carried out on the staining rack. In bacteriology, the Gram staining allows a fast differentiation of bacteria in Gram-positive and Gram-negative. The mureine structure of the bacteria wall is the basis of the color affinity. In the first step, bacteria will be stained with crystal violet an aniline dye. After the treatment with iodine solution (Lugol’s solution), a dye-iodine complex will form. During the decolorizing step, this complex stays in the multilayer mureine structure of the cell wall of Gram-positive bacteria - they will appear blue-violet. Gram-negative bacteria, by contrast, have a cell wall consisting of a single-layered murein structure, and correspondingly re-release the staining dye with the decoloring solution. Gram-negative bacteria will be counterstained with fuchsin solution and will then appear red.
The kit is sufficient for up to 65-70 applications. This product is registered as IVD and CE marked. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.
References
Product Information
HS Code3212 90 90
Quality LevelMQ400
Applications
Application(phenol-free) staining kit for Gram staining method on bacteriological smears
Biological Information
Physicochemical Information
Flash point-10 °C
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Hazard Pictogram(s) Hazard Pictogram(s)
Hazard Pictogram(s)
Hazard Pictogram(s)
Hazard Pictogram(s)
Hazard Statement(s)H225: Highly flammable liquid and vapour.
H318: Causes serious eye damage.
H336: May cause drowsiness or dizziness.
H351: Suspected of causing cancer.
H412: Harmful to aquatic life with long lasting effects.
Precautionary Statement(s)P202: Do not handle until all safety precautions have been read and understood.
P210: Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
P273: Avoid release to the environment.
P280: Wear protective gloves/ protective clothing/ eye protection/ face protection/ hearing protection.
P305 + P351 + P338: IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
P308 + P313: IF exposed or concerned: Get medical advice/ attention.
P403 + P233: Store in a well-ventilated place. Keep container tightly closed.
Signal WordDanger
Storage class3 Flammable liquids
WGKWGK 2 obviously hazardous to water
Safety Information
Product Usage Statements
Storage and Shipping Information
StorageStore at +15°C to +25°C.
Packaging Information
Transport Information
Declaration (railroad and road) ADR, RIDUN 3316 Entzündbarer flüssiger Stoff, n.a.g.(ACETON UND ENTH. ETHANOL), 9, II
Declaration (transport by air) IATA-DGRUN 3316 FLAMMABLE LIQUID, N.O.S.(ACETONE AND CONT. ETHANOL), 9, II
Declaration (transport by sea) IMDG-CodeUN 3316 FLAMMABLE LIQUID, N.O.S.(ACETONE AND CONT. ETHANOL), 9, II
Supplemental Information
Specifications
Suitability for microscopypasses test
gram-positve microorganismsdark blue
gram-negative microorganismsred
Global Trade Item Number
Catalogue Number GTIN
1016030001 04027269017330