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251520 DAF-FM DA - CAS 254109-22-3 - Calbiochem

A cell-permeable, photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~3 nM.

DAF-FM DA - CAS 254109-22-3 - Calbiochem MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: 3-Amino, 4-aminomethyl-2ʹ,7ʹ-difluorofluorescein Diacetate

251520
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Overview

Key Spec Table

CAS #Empirical Formula
254109-22-3C₂₅H₁₈F₂N₂O₇

Products

Catalogue NumberPackaging Qty/Pack
251520-1MG Glass bottle 1 mg
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Description
OverviewA cell-permeable, photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~3 nM. Intracellularly releases DAF-FM (Cat. No. 251515) by the action of esterases. Reacts with NO, in the presence of O2, resulting in a triazolo-fluorescein analog (DAF-FM T) that exhibits about 160-fold greater fluorescence quantum efficiency. The fluorescence is reported to be stable above pH 5.8.
Catalogue Number251520
Brand Family Calbiochem®
Synonyms3-Amino, 4-aminomethyl-2ʹ,7ʹ-difluorofluorescein Diacetate
References
ReferencesKojima, H., et al. 2001. J. Neurochem. 76, 1404.
Park. J.M., et al. 2001. Br. J. Pharmacol. 132, 1876.
Itoh, Y., et al. 2000. Anal. Biochem. 287, 203.
Kojima, H., et al. 1999. Angew Chem. Int. Ed. 38, 3209.
Product Information
CAS number254109-22-3
ATP CompetitiveN
DeclarationManufactured by Daiichi Pure Chemicals Co., Ltd
FormPale yellow solution
FormulationA 5 mM solution in DMSO.
Hill FormulaC₂₅H₁₈F₂N₂O₇
Chemical formulaC₂₅H₁₈F₂N₂O₇
ReversibleN
Structure formula ImageStructure formula Image
Quality LevelMQ100
Biological Information
Primary TargetNitric oxide (NO) fluorescent indicator
Purity≥98% by HPLC
Physicochemical Information
Cell permeableY
Emission max.
Excitation max.
Safety Information
R PhraseR: 36/38

Irritating to eyes and skin.
S PhraseS: 26

In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Irritant
Storage -20°C
Protect from Light Protect from light
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze at (-20°C). Use immediately after dilution; discard any unused portion.
Global Trade Item Number
Catalogue Number GTIN
251520-1MG 04055977198515