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SCC290 D425 Med Human Medulloblastoma Cell Line

D425 Med human medulloblastoma cell line is representative of a common aggressive form of medulloblastoma and is a well-established disease model.

SCC290
≥1X10⁶ cells/vial   
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberSCC290
DescriptionD425 Med Human Medulloblastoma Cell Line
Alternate Names
  • D-425 Med
  • D-425Med
  • D425Med
  • D-425
  • D425
  • Med 425
Background InformationMedulloblastoma is one of the most common malignant brain tumors in children, with the highest number of diagnoses occurring in patients between 6 and 8 years of age (1). Medulloblastomas are subdivided into four subtypes based on molecular genetic characteristics: WNT, sonic hedgehog (SHH), group 3 and group 4 (1). Of these, group 3 is characterized by amplification of the oncogene MYC, one of the most common gene alterations associated with medulloblastoma.

The D425 Med human medulloblastoma cell line is one of the most highly cited cellular models for medulloblastoma and is classified as a subgroup 3, harboring MYC amplification as well as mutant P53 (2). The D425 Med medulloblastoma cell line was derived from tumor cells of 6-year old patient and grows as suspended or lightly-adherent cell clumps in culture. D425 Med medulloblastoma cells are positive for neurofilament proteins but negative for GFAP (3).

Source:
The D425 medulloblastoma cell line was derived from biopsy of a tumor from a 6-year-old male patient (2).

References:
1. Nat Rev Dis Primers. 2019; 5(1): 11.
2. Lab Invest. 1991; 64(6): 833-43.
3. J Biotechnol. 2016; 236: 10-25.
References
Product Information
Applications
ApplicationD425 Med human medulloblastoma cell line is representative of a common aggressive form of medulloblastoma and is a well-established disease model.
Key Applications
  • Cell Culture
Application NotesThis product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the “Academic Use Agreement” as detailed in the product documentation. For information regarding any other use, please contact licensing@emdmillipore.com.
Biological Information
Cell Line Type
  • Cancer Cells
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance• Each vial contains ≥ 1X10⁶ viable cells.
• Cells are tested negative for infectious diseases by a Human Essential CLEAR panel by Charles River Animal Diagnostic Services.
• Cells are verified to be of human origin and negative for inter-species contamination from rat, mouse, chinese hamster, Golden Syrian hamster, and non-human primate (NHP) as assessed by a Contamination CLEAR panel by Charles River Animal Diagnostic Services.
• Cells are negative for mycoplasma contamination.
• Each lot of cells is genotyped by STR analysis to verify the unique identity of the cell line.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.
Packaging Information
Material Size≥1X10⁶ cells/vial
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
SCC290 04061842104024