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17-608 ChIPAb+ HDAC1 - ChIP Validated Antibody and Primer Set

17-608
10 assays  10 assays per kit, ~4μL per chromatin immunoprecipitation
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Overview

Key Spec Table

Species ReactivityKey Applications
H, MWB, ChIP
Description
Catalogue Number17-608
Brand Family Upstate
Trade Name
  • ChIPAb+
  • Upstate
DescriptionChIPAb+ HDAC1 - ChIP Validated Antibody and Primer Set
OverviewAll ChIPAb+ antibodies are individually validated for chromatin precipitation, every lot, every time. Each ChIPAb+ antibody set includes control primers (tested every lot by qPCR) to biologically validate your IP results in a locus-specific context. The qPCR protocol and primer sequences are provided, allowing researchers to validate ChIP protocols when using our antibody in their chromatin context. Each set also includes a negative control antibody to ensure specificity of the ChIP reaction.
The ChIPAb+ HDAC1 set includes the HDAC1 antibody, the negative control antibody supernatant (mouse IgG), and qPCR primers flanking an Sp1 binding site in human p21 (WAF1/CIP1/CDKN1A) promoter, amplifying a 105 base pair PCR product. The HDAC1 and negative control antibodies are supplied in a scalable "per ChIP" reaction size and can be used to functionally validate the precipitation of HDAC1 associated chromatin.
Product Information
FormatCulture Supernatant
Control
  • Included negative control antibody supernatant mouse IgG and control primers specific for human p21 (WAF1/CIP1/CDKN1A) promoter.
PresentationAnti-HDAC1 (mouse monoclonal IgG1,supernatant). One vial containing 40 μL of culture supernatant with 0.05% sodium azide. Store at -20°C.

Negative ChIP Control Supernatant. One vial containing 40 uL of mouse IgG containing supernatant with 0.05% sodium azide. Store at -20°C.

ChIP Primers p21. One vial containing 75 μL of 5 μM of each primer specific for a region of the human p21 (WAF1/CIP1/CDKN1A) promoter. Store at -20°C.
P21 Forward: CCC ACA GCA GAG GAG AAA GAA
P21 Reverse: CTG GAA ATC TCT GCC CAG ACA
Quality LevelMQ100
Applications
ApplicationThis ChIPAb+ HDAC1 -ChIP Validated Antibody & Primer Set conveniently includes the antibody & the specific control PCR primers.
Key Applications
  • Western Blotting
  • Chromatin Immunoprecipitation (ChIP)
Application NotesChromatin Immunoprecipitation Analysis:
Sonicated chromatin prepared from untreated or actinomycin D treated (30 ng/mL, 6 hrs.) U2OS cells (3 X 106 cell equivalents per IP) was subjected to chromatin immunoprecipitation using 4 μL of Mouse Anti-HDAC1 and the Magna ChIP™ G (Cat. # 17-611) Kit. Immunoprecipitation of HDAC1 associated DNA fragments was verified by qPCR using ChIP Primers p21 flanking the human p21 promoter that contains an Sp1 binding site (Please see figures).
Fold Decrease is a ratio of normalized mean IP quantities extracted from standard curves derived from inputs of each chromatin sample. As previously published, HDAC1 association with this region of the p21 promoter decreases upon actinomycin D treatment.

Western Blot Analysis:
3T3/A31 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-HDAC1 (1:2500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system (Please see figures).
Biological Information
ImmunogenThe HDAC1 antibody is made against a peptide corresponding to amino acids 467-482 of mouse HDAC1.
HostMouse
SpecificityHDAC1
IsotypeIgG1
Species Reactivity
  • Human
  • Mouse
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • HD1
  • HDAC1
  • RPD3L1
UniProt Number
Molecular Weight60 kDa
Product Usage Statements
Quality AssuranceRoutinely evaluated by chromatin immunoprecipitation on U2OS cells and immunoblot on 3T3/A31 nuclear lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt
Packaging Information
Material Size10 assays
Material Package10 assays per kit, ~4μL per chromatin immunoprecipitation
Global Trade Item Number
Catalogue Number GTIN
17-608 04053252371011