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219007 Caspase Inhibitor VI - CAS 161401-82-7 - Calbiochem

Z-VAD-FMK, CAS 161401-82-7, is an irreversible pan caspase inhibitor. Does not require pretreatment with esterase for in vitro studies.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Z-VAD-FMK

Primary Target: general caspase inhibitor
219007
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

CAS #Empirical Formula
161401-82-7C₂₁H₂₈FN₃O₇

Products

Catalogue NumberPackaging Qty/Pack
219007-1MG Plastic ampoule 1 mg
219007-250UG Plastic ampoule 250 μg
Description
OverviewAn irreversible general caspase inhibitor. Useful for studies involving recombinant, isolated, and purified caspase enzymes. Unlike Caspase Inhibitor I (Cat. No. 627610), this inhibitor does not require pretreatment with esterase for in vitro studies. A 10 mM (1 mg/221 µl) solution of Caspase Inhibitor VI (Cat. No. 219011) in DMSO is also available.
Catalogue Number219007
Brand Family Calbiochem®
SynonymsZ-VAD-FMK
References
ReferencesMisaghi, S., et al. 2004. Chem. Biol. 11, 1677.
Product Information
CAS number161401-82-7
ATP CompetitiveN
FormOff-white solid
Hill FormulaC₂₁H₂₈FN₃O₇
Chemical formulaC₂₁H₂₈FN₃O₇
ReversibleN
Structure formula ImageStructure formula Image
Quality LevelMQ100
Applications
Biological Information
Primary Targetgeneral caspase inhibitor
Purity≥98% by HPLC
Physicochemical Information
Cell permeableN
Peptide SequenceZ-Val-Ala-Asp-CH₂F*
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage -20°C
Protect from Moisture Protect from moisture
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
219007-1MG 07790788048648
219007-250UG 04055977202021

Documentation

Caspase Inhibitor VI - CAS 161401-82-7 - Calbiochem Certificates of Analysis

TitleLot Number
219007

References

Reference overview
Misaghi, S., et al. 2004. Chem. Biol. 11, 1677.

Brochure

Title
Caspases and other Apoptosis Related Tools Brochure

Citations

Title
  • Liwei Chen, et al. (2005) Chemical ablation of androgen receptor in prostate cancer cells by the histone deacetylase inhibitor LAQ824. Molecular Cancer Therapeutics 4, 1311-1319.
  • Heui-Young Ryu, et al. (2005) Environmental Chemical-Induced Bone Marrow B Cell Apoptosis: Death Receptor-Independent Activation of a Caspase-3 to Caspase-8 Pathway. Molecular Pharmacology 68, 1087-1096.
  • Yumin Zhang, et al. (2004) Peroxynitrite-Induced Neuronal Apoptosis is Mediated by Intracellular Zinc Release and 12-Lipoxygenase Activation. Journal of Neuroscience 24, 10616-10627.
  • Dong Zhang, et al. (2001) Granzymes A and B Directly Cleave Lamins and Disrupt the Nuclear Lamina During Granule-Mediated Cytolysis. Proceedings of the National Academy of Science USA 98, 5746-5751.
  • Brian L. Webb, Elsa Jimenez and G. Steven Martin. (2000) v-Src Generates a p53-Independent Apoptotic Signal. Molecular and Cellular Biology 20, 9271-9280.
    		•
    Data Sheet

    Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

    Revision17-July-2014 JSW
    SynonymsZ-VAD-FMK
    DescriptionA cell-permeable, irreversible, and general caspase inhibitor. Also reported to inhibit Peptide: N-glycanase (PNGase) in vitro and in vivo. Useful for studies involving recombinant, isolated, and purified caspase enzymes. Unlike Caspase Inhibitor I (Cat. No. 627610), this inhibitor does not require pretreatment with esterase for in vitro studies.
    FormOff-white solid
    CAS number161401-82-7
    Chemical formulaC₂₁H₂₈FN₃O₇
    Peptide SequenceZ-Val-Ala-Asp-CH₂F*
    Structure formulaStructure formula
    Purity≥98% by HPLC
    SolubilityDMSO (9 mg/ml)
    Storage Protect from moisture
    -20°C
    Do Not Freeze Ok to freeze
    Special InstructionsFollowing reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
    Toxicity Standard Handling
    ReferencesMisaghi, S., et al. 2004. Chem. Biol. 11, 1677.
    Citation
  • Liwei Chen, et al. (2005) Chemical ablation of androgen receptor in prostate cancer cells by the histone deacetylase inhibitor LAQ824. Molecular Cancer Therapeutics 4, 1311-1319.
  • Heui-Young Ryu, et al. (2005) Environmental Chemical-Induced Bone Marrow B Cell Apoptosis: Death Receptor-Independent Activation of a Caspase-3 to Caspase-8 Pathway. Molecular Pharmacology 68, 1087-1096.
  • Yumin Zhang, et al. (2004) Peroxynitrite-Induced Neuronal Apoptosis is Mediated by Intracellular Zinc Release and 12-Lipoxygenase Activation. Journal of Neuroscience 24, 10616-10627.
  • Dong Zhang, et al. (2001) Granzymes A and B Directly Cleave Lamins and Disrupt the Nuclear Lamina During Granule-Mediated Cytolysis. Proceedings of the National Academy of Science USA 98, 5746-5751.
  • Brian L. Webb, Elsa Jimenez and G. Steven Martin. (2000) v-Src Generates a p53-Independent Apoptotic Signal. Molecular and Cellular Biology 20, 9271-9280.
    		•