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14-741M CHK2 (I157T), active

CHK2 (I157T), active MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
14-741M
250 µg  Also available in 10μg size as catalog # 14-741 and in 250 μg as catalog # 14-741M.
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue Number14-741M
Trade Name
  • Upstate
DescriptionCHK2 (I157T), active
OverviewSerine/threonine-protein kinase which is required for checkpoint-mediated cell cycle arrest, activation of DNA repair and apoptosis in response to the presence of DNA double-strand breaks. May also negatively regulate cell cycle progression during unperturbed cell cycles. Following activation, phosphorylates numerous effectors preferentially at the consensus sequence [L-X-R-X-X-S/T]. Regulates cell cycle checkpoint arrest through
phosphorylation of CDC25A, CDC25B and CDC25C, inhibiting their activity. Inhibition of CDC25 phosphatase activity leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes and blocks cell cycle progression. May also phosphorylate NEK6 which is involved in G2/M cell cycle arrest. Regulates DNA repair through phosphorylation of BRCA2, enhancing the association of RAD51 with chromatin which promotes DNA repair by homologous recombination. Also stimulates the transcription of genes involved in DNA repair (including BRCA2) through the phosphorylation and activation of the transcription factor FOXM1. Regulates apoptosis through the phosphorylation of p53/TP53, MDM4 and PML. Phosphorylation of p53/TP53 at 'Ser-20' by CHEK2 may alleviate inhibition by MDM2, leading to accumulation of active p53/TP53. Phosphorylation of MDM4 may also reduce degradation of p53/TP53. Also controls the transcription of pro-apoptotic genes through phosphorylation of the transcription factor E2F1. Tumor suppressor, it may also have a DNA damage-independent function in mitotic spindle assembly by phosphorylating BRCA1. Its absence may be a cause of the chromosomal instability observed in some cancer cells
References
Product Information
PresentationProtein in in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
Quality LevelMQ100
Applications
Key Applications
  • Kinase Assay
Biological Information
SourceN-terminal GST and C-terminal 6His-tagged,.recombinant human CHK2, residues 5 - end, containing the I157T mutation. Expressed in E. coli cells
Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Entrez Gene Number
Gene Symbol
  • CHEK2
  • CHK2
  • RAD53
  • CDS1
  • LFS2
Protein TargetCHK2
Purification MethodPurified using glutathione-agarose and Ni2+/NTA-agarose.
Target Sub-FamilyCAMK
UniProt Number
Molecular Weight89.6 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceRoutinely evaluated by phosphorylation of CHKtide (KKKVSRSGLYRSPSMPENLNRPR)
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions12 months at -70°C
Packaging Information
Material Size250 µg
Material PackageAlso available in 10μg size as catalog # 14-741 and in 250 μg as catalog # 14-741M.
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
14-741M 04053252411922

Related Products & Applications

Alternative Packsize

Catalogue Number Description
14-741 CHK2 (I157T), active

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Kinase & Phosphatase Products

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Categories

Life Science Research > Proteins and Enzymes > Purified Kinases
Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases