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100068 MilliporeBOLTON broth (base)

acc. ISO 10272 GranuCult® prime

BOLTON broth (base) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

SDS
CoA
Data Sheet
User Guides

ph Value: 7.2 - 7.6 (27.6 g/l, H₂O, 25 °C) (after autoclaving) Solubility: 27.6 g/l

Recommended Products

Overview

Replacement Information

Key Spec Table

ph Value	Solubility
7.2 - 7.6 (27.6 g/l, H₂O, 25 °C) (after autoclaving)	27.6 g/l

Products

Catalogue Number	Packaging	Qty/Pack
1000680500	Plastic bottle	500 g	Add To Favorites Add To Favorites Added To My Favorites

Description
Catalogue Number	100068
Overview	GranuCult™ is our new brand name for our existing granulated dehydrated culture media which apply to new EN ISO 11133:2014 and various other ISO standards and FDA-BAM / USDA-FSIS methods for testing of food and water samples. All these media are currently being updated to be fully compliant to the latest version of these standards and regulations with respect to composition and quality control and will be available shortly.

Description

Catalogue Number

100068

Overview

GranuCult™ is our new brand name for our existing granulated dehydrated culture media which apply to new EN ISO 11133:2014 and various other ISO standards and FDA-BAM / USDA-FSIS methods for testing of food and water samples. All these media are currently being updated to be fully compliant to the latest version of these standards and regulations with respect to composition and quality control and will be available shortly.

References

Product Information
HS Code	3821 00 00
Quality Level	MQ200

Applications

Biological Information

Physicochemical Information
pH value	7.2 - 7.6 (27.6 g/l, H₂O, 25 °C) (after autoclaving)
Solubility	27.6 g/l

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS
Hazard Pictogram(s)
Hazard Statement(s)	H317: May cause an allergic skin reaction. H318: Causes serious eye damage.
Precautionary Statement(s)	P261: Avoid breathing dust. P272: Contaminated work clothing should not be allowed out of the workplace. P280: Wear protective gloves/ eye protection/ face protection. P302 + P352: IF ON SKIN: Wash with plenty of water. P305 + P351 + P338: IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. P333 + P313: If skin irritation or rash occurs: Get medical advice/ attention.
Signal Word	Danger
Storage class	10 - 13 Other liquids and solids
WGK	WGK 3 highly hazardous to water
Disposal	3 Relatively unreactive organic reagents should be collected in container A. If halogenated, they should be collected in container B. For solid residues use container C.

Safety Information
Categories of danger	irritant

Product Usage Statements

Storage and Shipping Information
Storage	Store at +15°C to +25°C.

Packaging Information

Transport Information

Supplemental Information

Specifications
Appearance (color)	dark red to black
pH-value (25 °C)	7.2 - 7.6
Growth promotion test in accordance with the current version of DIN EN ISO 11133. Mixed inoculum of Campylobacter, E.coli and P.mirabilis. Additionaly monoculture of E.coli and P.mirabilis. Afterwards confirmation on mCCDA resp. Tryptic soy agar.
Inoculum on reference medium (Campylobacter coli ATCC 43478 (WDCM 00004))	≤ 100
Inoculum on reference medium (Campylobacter jejuni ATCC 33291 (WDCM 00005))	≤ 100
Inoculum on reference medium (Campylobacter jejuni ATCC 29428 (WDCM 00156))	≤ 100
Inoculum on reference medium (Escherichia coli ATCC 25922 (WDCM 00013))	≥ 10000
Inoculum on reference medium (Escherichia coli ATCC 8739 (WDCM 00012))	≥ 10000
Inoculum on reference medium (Proteus mirabilis ATCC 29906 (WDCM 00023))	≥ 10000
Growth on mCCDA (Campylobacter coli ATCC 43478 (WDCM 00004))	> 10 CFU
Growth on mCCDA (Campylobacter jejuni ATCC 33291 (WDCM 00005))	> 10 CFU
Growth on mCCDA (Campylobacter jejuni ATCC 29428 (WDCM 00156))	> 10 CFU
Growth on Tryptic soy agar (Escherichia coli ATCC 25922 (WDCM 00013))	no growth
Growth on Tryptic soy agar (Escherichia coli ATCC 8739 (WDCM 00012))	no growth
Growth on Tryptic soy agar (Proteus mirabilis ATCC 29906 (WDCM 00023))	no growth
Colony color (Campylobacter coli ATCC 43478 (WDCM 00004))	greyish
Colony color (Campylobacter jejuni ATCC 33291 (WDCM 00005))	greyish
Colony color (Campylobacter jejuni ATCC 29428 (WDCM 00156))	greyish
Incubation: Bolton broth: 5 ± 1 hours at 37 ± 1 °C, afterwards 44 ± 4 hours at 41.5 ± 1 °C, Anaerocult C Confirmation on mCCDA: at least 44 ± 4 hours at 41.5 ± 1 °C, Anaerocult C Confirmation on Tryptic Soy Agar: 24 ± 3 hours at 37 ± 1 °C, aerobic

Global Trade Item Number
Catalogue Number	GTIN
1000680500	04022536977331

Documentation

BOLTON broth (base) SDS

Title
Safety Data Sheet (SDS)

BOLTON broth (base) Certificates of Analysis

Product Number	Packaging	Specification	Lot Number
1000680500	Plastic bottle	PDF Specification Document	Enter Lot Number

Data Sheet

Title
Data Sheet 1.00068.0500

User Guides

Title
Pack insert Bolton Broth Selective Supplement 1.00079.0010

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100068 MilliporeBOLTON broth (base)

acc. ISO 10272 GranuCult® prime

Recommended Products

Overview

Key Spec Table

Products

Documentation

BOLTON broth (base) SDS

BOLTON broth (base) Certificates of Analysis

Data Sheet

User Guides

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