Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

05-1923 Anti-phospho-Threonine Antibody, clone 20H6.1

This phospho-Threonine antibody is validated for use in WB & ICC for the detection of the phospho-Threonine protein.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: General Post-translation Modification Gene Symbol: THR
05-1923
100 µg  
Purchase on Sigma-Aldrich

Special Offers

Overview

Replacement Information

Special Offers

100% Performance Guaranteed

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, M, AWB, ICCMPurifiedMonoclonal Antibody
Description
Catalogue Number05-1923
DescriptionAnti-phospho-Threonine Antibody, clone 20H6.1
Alternate Names
  • Threonine
Background InformationProtein phosphorylation is normally associated with protein modification in signaling pathways and may be useful in pathophysiology and treatment of major neuropsychiatric disorders. Antibodies against phospho-threonine have been used to establish stability of the phosphorylated forms of proteins that may be involved in many different types of signaling. Phospho-threonine antibody is used to measure phosphorylation levels of PSII reaction center proteins D1, D3 and chlorophyll a binding protein CP43.
References
Product Information
FormatPurified
Control
  • Calyculin/okadaic acid treated and untreated A431 cell lysates.
PresentationPurified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis phospho-Threonine antibody is validated for use in WB & ICC for the detection of the phospho-Threonine protein.
Key Applications
  • Western Blotting
  • Immunocytochemistry
Application NotesImmunoprecipitation Analysis: 10 µg of this antibody immunoprecipitated Threonine from 100 µg of A431 cells stimulated with calyculin/okadaic acid.

Immunocytochemistry Analysis: 1:500 dilution from a previous lot detected Threonine in A431, NIH/3T3, and HeLa cells.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to Threonine.
Clone20H6.1
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
IsotypeIgG2bκ
Species Reactivity
  • Human
  • Mouse
  • All
Species Reactivity NoteDemonstrated to react with human and mouse. Predicted to react with all vertebrates based on 100% sequence homology.
Antibody TypeMonoclonal Antibody
Gene Symbol
  • THR
Purification MethodProtein G
Molecular WeightVarious observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in calyculin/okadaic acid treated and untreated A431 cell lysates.

Western Blot Analysis: 0.5 µg/mL of this antibody detected Threonine on 10 µg of calyculin/okadaic acid treated and untreated A431 cell lysates.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
05-1923 04053252629785

Documentation

Anti-phospho-Threonine Antibody, clone 20H6.1 SDS

Title

Safety Data Sheet (SDS) 

Anti-phospho-Threonine Antibody, clone 20H6.1 Certificates of Analysis

TitleLot Number
Anti-phospho-Threonine, clone 20H6.1 - 2390575 2390575
Anti-phospho-Threonine, clone 20H6.1 - 2006963 2006963
Anti-phospho-Threonine, clone 20H6.1 - 2092738 2092738
Anti-phospho-Threonine, clone 20H6.1 - 2179361 2179361
Anti-phospho-Threonine, clone 20H6.1 - 2297321 2297321
Anti-phospho-Threonine, clone 20H6.1 - 3198422 3198422
Anti-phospho-Threonine, clone 20H6.1 - 4005439 4005439
Anti-phospho-Threonine, clone 20H6.1 - 4020604 4020604
Anti-phospho-Threonine, clone 20H6.1 - NG1939322 NG1939322
Anti-phospho-Threonine, clone 20H6.1 - NRG1854605 NRG1854605
Show more