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MABF934-25UL Anti-phospho-SAMHD1 (Thr592) Antibody, clone T592P

Anti-phospho-SAMHD1 (Thr592), clone T592P, Cat. No. MABF934 is a mouse monoclonal antibody that detects SAMHD1 and has been tested for use in Flow Cytometry and Western Blotting.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology Gene Symbol: SAMHD1, MOP5 UniProt Number: Q9Y3Z3
MABF934-25UL
25 µL  
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Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HFC, WBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABF934-25UL
DescriptionAnti-phospho-SAMHD1 (Thr592) Antibody, clone T592P
Alternate Names
  • Deoxynucleoside triphosphate triphosphohydrolase SAMHD1
  • dNTPase
  • Dendritic cell-derived IFNG-induced protein
  • DCIP
  • Monocyte protein 5
  • MOP-5
  • SAM domain and HD domain-containing protein 1
Background InformationDeoxynucleoside triphosphate triphosphohydrolase SAMHD1 (UniProt: Q9Y3Z3; also known as dNTPase, Dendritic cell-derived IFNG-induced protein, DCIP, Monocyte protein 5, MOP-5, SAM domain and HD domain-containing protein 1) is encoded by the SAMHD1 (also known as MPOS) gene (Gene ID: 25939) in human. SAMHD1 is a nuclear protein that functions as a host restriction nuclease that blocks early-stage virus replication in dendritic and other myeloid cells. It suppresses LINE-1 retrotransposon activity and may function by reducing the cellular dNTP to such low levels that prevents retroviral reverse transcription. SAMHD1 is also reported to play a role in mediating pro-inflammatory responses to TNF-alpha signaling. It is expressed in heart, skeletal muscle, spleen, liver, small intestine, placenta, lung and peripheral blood leukocytes, however, no expression is seen in brain and thymus tissue. Its expression is shown to be upregulated in TNF-alpha treated fibroblasts. SAMHD1 is allosterically stimulated by dGTP, which binds in a cleft at the interface of the homodimer and promotes the formation of highly active homotetramers. Four isoforms of SAMHD1 have been described that are produced by alternate splicing. Mutations in SAMHD1 gene are reported to be involved in the pathogenesis of Chilblain lupus 2, a cutaneous form of lupus erythematosus and in Aicardi-Goutieres syndrome 5 that is characterized by cerebral atrophy, leukoencephalopathy, intracranial calcifications, chronic cerebrospinal fluid (CSF) lymphocytosis.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
ApplicationAnti-phospho-SAMHD1 (Thr592), clone T592P, Cat. No. MABF934 is a mouse monoclonal antibody that detects SAMHD1 and has been tested for use in Flow Cytometry and Western Blotting.
Key Applications
  • Flow Cytometry
  • Western Blotting
Application NotesFlow Cytometry Analysis: A representative lot detected phospho-SAMHD1 (Thr592) in Flow Cytometry applications (Coiras, M., et. al. (2016). Cell Rep. 14(9):2100-7).

Western Blotting Analysis: A representative lot detected phospho-SAMHD1 (Thr592) in Western Blotting applications (Cribier, A., et. al. (2013). Cell Rep. 3(4):1036-43; Calantone, N., et. al. (2014). Immunity. 41(3):493-502; Coiras, M., et. al. (2016). Cell Rep. 14(9):2100-7).
Biological Information
ImmunogenA linear peptide corresponding to 11 amino acids surrounding phosphoryalted Thr592 from the C-terminal region of huamn SAMHD1.
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone T592P detects Deoxynucleoside triphosphate triphosphohydrolase SAMHD1 in human cells. It targets an epitope with in 11 amino acids surrounding the phosphorylated Thr592 from the C-terminal region.
IsotypeIgG2a
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Gene Symbol
  • SAMHD1
  • MOP5
Purification MethodProtein G purified
UniProt Number
Molecular Weight~72 kDa observed; 72.20 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in CD4+ T lymphocytes (activated with PMA/IL-2) lysates.

Western Blotting Analysis: A 1:500 dilution of this antibody detected phospho-SAMHD1 (Thr592) in CD4+ T lymphocytes (activated with PMA/IL-2) lysates.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size25 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABF934-25UL 04054839494154