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ABS2231-25UG Anti-phospho RyR2 (Ser2808)

Anti-phospho RyR2 (Ser2808), Cat. No. ABS2231, is a rabbit polyclonal antibody that detects RyR2 phosphorylated on serine 2808 and is tested for use in Western Blotting.

ABS2231-25UG
25 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberABS2231-25UG
DescriptionAnti-phospho RyR2 (Ser2808)
Alternate Names
  • RYR-2
  • RyR2
  • hRYR-2
  • Cardiac muscle ryanodine receptor
  • Cardiac muscle ryanodine receptor-calcium release channel
  • Type 2 ryanodine receptor
Background InformationRyanodine receptor 2 (UniProt: Q92736; also known as RYR-2, RyR2, hRYR-2, Cardiac muscle ryanodine receptor, Cardiac muscle ryanodine receptor-calcium release channel, Type 2 ryanodine receptor) is encoded by the RYR2 gene (Gene ID: 6262) in human. Ryanodine receptors are large ion channels that are located in the sarcoplasmic/endoplasmic reticulum membrane and are responsible for the release of Ca2+. They form homotetramers with a mushroom-like shape, consisting of a large cytoplasmic head and transmembrane stalk. In mammals three isoforms of RYR have been described (RYR 1-3) that are regulated by phosphorylation, redox modifications, and a variety of small proteins, and ions. Ryanodine receptor 2 is a multi-pass membrane protein with two cytoplasmic domains (aa 1-4281 and 4871-4967), six transmembrane domains, and one pore-forming domain (aa 4820-4829). It is the predominant form in cardiac muscle and is also expressed in Purkinje cells of cerebellum and cerebral cortex. It plays a key role in triggering cardiac muscle contraction and aberrant channel activation can lead to cardiac arrhythmia. Channel activity is modulated by ryanodine that binds to the open Ca2+-release channel with high affinity. At low concentrations, ryanodine maintains the channel in an open conformation, but high concentrations of ryanodine inhibit channel activity. Channel activity is also modulated by phosphorylation. Phosphorylation at serine 2808 and serine 2814 is shown to increase the open probability of the calcium channel. Phosphorylation is increased in failing heart, leading to calcium leaks, and increased cytoplasmic Ca2+ levels. (Ref.: Xiao, Z., et al. (2016). J. Biol. Chem. 291(47); 24528-24537; Lanner, JT., et al. (2010). Cold Spring Harb. Perspect. Biol. 2(11); a003996).
References
Product Information
FormatPurified
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl without azide
Quality LevelMQ200
Applications
ApplicationAnti-phospho RyR2 (Ser2808), Cat. No. ABS2231, is a rabbit polyclonal antibody that detects RyR2 phosphorylated on serine 2808 and is tested for use in Western Blotting.
Key Applications
  • Western Blotting
Application NotesNote: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenA KLH-conjugated linear peptide corresponding to 8 amino acids surrounding phosphoserine 2808 from human Ryanodine receptor 2.
EpitopeC-terminal cytoplasmic domain
Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostRabbit
SpecificityThis rabbit polyclonal antibody detects Ryanodine receptor 2 (RyR2) phosphorylated on serine 2808.
IsotypeIgG
Species Reactivity
  • Rabbit
Species Reactivity NoteRabbit. Predicted to react with Human, Mouse based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • RYR2
Modifications
  • Phosphorylation
Purification MethodAffinity Purified
UniProt Number
Molecular Weight~565 kDa observed; 564.57 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in rabbit skeleton muscle microsomal preparation.

Western Blotting Analysis (WB): A 1:300 dilution of this antibody detected RyR2 phosphorylated on serine 2808 in rabbit skeleton muscle microsomal preparations, but not in microsomes treated with protein phosphatase.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABS2231-25UG 04065269135128