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MABN2431-100UL Anti-phospho-NFL (Ser473) Antibody, clone 4F8

Anti-phospho-NFL (Ser473), clone 4F8, Cat. No. MABN2431, is a highly specific mouse monoclonal antibody that targets Neurofilament light polypeptide phosphorylated on Ser473 and has been tested for use in Immunohistochemistry and Western Blotting.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Gene Symbol: NEFL, NF68, NFL UniProt Number: P07196
MABN2431-100UL
100 µL  
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Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, MIHC, WBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABN2431-100UL
DescriptionAnti-phospho-NFL (Ser473) Antibody, clone 4F8
Alternate Names
  • Neurofilament light polypeptide
  • 68 kDa neurofilament protein
  • Neurofilament triplet L protein
Background InformationNeurofilament light polypeptide (UniProt: P07196; also known as NF-L, 68 kDa neurofilament protein, Neurofilament triplet L protein) is encoded by the NEFL (also known as NF68, NFL) gene (Gene ID: 4747) in human. Neurofilaments are a type of intermediate filament that serve as major elements of the cytoskeleton supporting the axon cytoplasm. They are the most abundant fibrillar components of the axon. Although typically restricted to neurons, neurofilaments have been detected in paragangliomas and in adrenal and extra-adrenal pheochromocytomas. Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. The neurofilament triplet proteins (68/70, 160, and 200 kDa) occur in both the central and peripheral nervous system and are usually neuron specific. The 68/70 kDa NF-L protein can self-assemble into a filamentous structure, however the 160 kDa NF-M and 200 kDa NF-H proteins require the presence of the 68/70 kDa NF-L protein to co-assemble. Mutations in NEFL gene can lead to Charcot-Marie-Tooth disease 1F and 2E disease that is characterized by demyelinating and progressive weakness and atrophy, initially of the peroneal muscles and later of the distal muscles of the arms.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide
Quality LevelMQ100
Applications
ApplicationAnti-phospho-NFL (Ser473), clone 4F8, Cat. No. MABN2431, is a highly specific mouse monoclonal antibody that targets Neurofilament light polypeptide phosphorylated on Ser473 and has been tested for use in Immunohistochemistry and Western Blotting.
Key Applications
  • Immunohistochemistry
  • Western Blotting
Application NotesImmunohistochemistry Analysis: A representative lot detected phospho-NFL (Ser473) in Immunohistochemistry applications (Rutherford, N.J., et. al. (2016). Acta Neuropathol Commun. 4(1):80).

Western Blotting Analysis: A representative lot detected phospho-NFL (Ser473) in Western Blotting applications (Rutherford, N.J., et. al. (2016). Acta Neuropathol Commun. 4(1):80).
Biological Information
ImmunogenKLH-conjugted linear peptide corresponding to 15 amino acids surrounding phosphorylated serine 473. Cystine was added for conjugation to KLH.
Clone4F8
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 4F8 specifically detects Neurofilament light polypeptide phosphorylated on Serine 473 in human and murine cells.
IsotypeIgG1κ
Species Reactivity
  • Human
  • Mouse
Species Reactivity NoteHuman, Mouse. Predicted to react with Porcine, Rhesus Macaque, Bovine, Rat based on 100% sequence homology.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • NEFL
  • NF68
  • NFL
Modifications
  • Phosphorylation
Purification MethodProtein G purified
UniProt Number
Molecular Weight~70 kDa observed; 61.52 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in mouse brain tissue lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected phospho-NFL (Ser473) in mouse brain tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABN2431-100UL 04054839431777