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ABE1298-100UL Anti-phospho BAF60C (Ser247)

Anti-phospho BAF60C (Ser247), Cat. No. ABE1298, is a highly specific rabbit polyclonal antibody that targets SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily D member 3 (BAF60C) phosphorylatd on Ser247 and has been tested for use in Peptide Inhibition Assay & WB.

Research Category: Epigenetics & Nuclear Function Gene Symbol: SMARCD3, BAF60C UniProt Number: Q6STE5
ABE1298-100UL
100 µL  
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Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
MWB, PIARbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABE1298-100UL
DescriptionAnti-phospho BAF60C (Ser247)
Alternate Names
  • SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily D member 3
  • 60 kDa BRG-1/Brm-associated factor subunit C
  • BRG1-associated factor 60C
  • BAF60C
Background InformationSWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily D member 3 (UniProt: Q6STE5; also known as 60 kDa BRG-1/Brm-associated factor subunit C, BRG1-associated factor 60C, BAF60C) is encoded by the SMARCD3 (also known as BAF60C) gene (Gene ID: 6604) in human. BAF60C plays a key role in ATP dependent nucleosome remodeling by SMARCA4 containing complexes. Two isoforms of BAF60C have been reported that are produced by alternative splicing. Both isoforms are expressed in brain, heart, kidney, placenta, prostate, salivary glands, spleen, testis, thyroid, trachea, and uterus. However, isoform 1 is expressed also in skeletal muscle and adipose tissue. BAF60C belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. BAF60C is also a component of nuclear Notch signaling and is required for the establishment of left-right asymmetry. Loss of BAF60C can lead to defects in the establishment of the left-right asymmetry cascade in mouse and zebrafish. It also serves a specific chromatin remodeling component for lipogenic gene transcription in liver. In response to insulin, it is phosphorylated at serine 247 by atypical PKC zeta//lambda, which causes translocation of BAF60C to the nucleus and allows a direct interaction of BAF60C with upstream stimulatory factor-1 (USF-1). (Ref.: Takeuchi, JK., et al. (2007). Proc. Natl. Acad. Sci. USA 104(3); 846-851; Wang, Y., et al. (2012). Mol. Cell 49(2); 283-297).
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-phospho BAF60C (Ser247), Cat. No. ABE1298, is a highly specific rabbit polyclonal antibody that targets SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily D member 3 (BAF60C) phosphorylatd on Ser247 and has been tested for use in Peptide Inhibition Assay & WB.
Key Applications
  • Western Blotting
  • Peptide Inhibition Assay
Application NotesPeptide Inhibition Analysis: A 1:500 dilution from a representative lot was used with NIH3T3 Nuclear Extract for peptide block analysis.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to 9 amino acids surrounding phosphorylated serine 247 of human BAF60C. The immunogen is conserved between both isoforms.
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis rabbit polyclonal antibody detects murine BAF60C phosphorylated on Serine 247.
Species Reactivity
  • Mouse
Species Reactivity NoteMouse. Predicted to react with Bovine, Human, and Rat based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • SMARCD3
  • BAF60C
Modifications
  • Phosphorylation
Purification MethodAffinity Purified
UniProt Number
Molecular Weight~55 kDa observed. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in NIH3T3 Nuclear Extract.

Western Blotting Analysis: A 1:500 dilution of this antibody detected phospho BAF60C (Ser247) in NIH3T3 Nuclear Extract.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABE1298-100UL 04054839448157

Documentation