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04-294 Anti-phosho-erbB2/Her2 (Tyr1112) Antibody, clone 19G5

Detect phosho-erbB2/Her2 (Tyr1112) using this Anti-phosho-erbB2/Her2 (Tyr1112) Antibody, clone 19G5 validated for use in ELISA & WB.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Growth Factors & Receptors Gene Symbol: ERBB2, HER2, HER-2, NGL, herstatin, erbB-2, C-erbB-2, NEU, TKR1, p185erbB2, HER-2/neu UniProt Number: P04626
04-294
100 µg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
Ca, H, M, RELISA, WBMPurifiedMonoclonal Antibody
Description
Catalogue Number04-294
Brand Family Upstate
Trade Name
  • Upstate
DescriptionAnti-phosho-erbB2/Her2 (Tyr1112) Antibody, clone 19G5
References
Product Information
FormatPurified
Control
  • Includes EGF treated SKOV-3 lysate as a positive control
PresentationLyophilized in 2X PBS containing 0.09% sodium azide, PEG, and sucrose.
Quality LevelMQ100
Applications
ApplicationDetect phosho-erbB2/Her2 (Tyr1112) using this Anti-phosho-erbB2/Her2 (Tyr1112) Antibody, clone 19G5 validated for use in ELISA & WB.
Key Applications
  • ELISA
  • Western Blotting
Biological Information
ImmunogenKLH-conjugated synthetic peptide encompassing the surrounding amino acids of Tyr1112 in human erbB2/Her2
Clone19G5
HostMouse
SpecificityRecognizes phosphorylated domain of erbB2/Her2 on Tyrosine 1112
IsotypeIgG1
Species Reactivity
  • Canine
  • Human
  • Mouse
  • Rat
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThis gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized.
Gene Symbol
  • ERBB2
  • HER2
  • HER-2
  • NGL
  • herstatin
  • erbB-2
  • C-erbB-2
  • NEU
  • TKR1
  • p185erbB2
  • HER-2/neu
Modifications
  • Phosphorylation
Purification MethodSubsequent thiophilic adsorption and size exclusion chromatography
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P04626 # Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Not activated by EGF, TGF- alpha and amphiregulin.
SIZE: 1255 amino acids; 137910 Da
SUBUNIT: Heterodimer with each of the other ERBB receptors (Potential). Interacts with PRKCABP and PLXNB1. Part of a complex with EGFR and either PIK3C2A or PIK3C2B. May interact with PIK3C2B when phosphorylated on Tyr-1196. Interacts with MEMO when phosphorylated on Tyr-1248.
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
PTM: Ligand-binding increases phosphorylation on tyrosine residues (By similarity).
SIMILARITY: SwissProt: P04626 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily. & Contains 1 protein kinase domain.
Molecular Weight185 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceRoutinely evaluated by immunoblot.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions2 years at -20°C from date of shipment. Reconstitute prior to use. Reconstitute with 1 mL H2O for 15 min at room temperature. Reconstituted aliquots should be stored frozen at -80°C. Thawed aliquots may be stored at 4°C for up to 3 months. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
04-294 04053252367526

Documentation

Anti-phosho-erbB2/Her2 (Tyr1112) Antibody, clone 19G5 Certificates of Analysis

TitleLot Number
Anti-phosho-erbB2/Her2 (Tyr1112), clone 19G5 (Mouse monoclonal IgG1) - 2454690 2454690
Anti-phosho-erbB2/Her2 (Tyr1112), clone 19G5 (Mouse monoclonal IgG1) 2967801
Anti-phosho-erbB2/Her2 (Tyr1112), clone 19G5 (Mouse monoclonal IgG1) 2943145
Anti-phosho-erbB2/Her2 (Tyr1112), clone 19G5 - 2383445 2383445
Anti-phosho-erbB2/Her2 (Tyr1112), clone 19G5 - 0216S0102 0216S0102
Anti-phosho-erbB2/Her2 (Tyr1112), clone 19G5 - 2914554 2914554
Anti-phosho-erbB2/Her2 (Tyr1112), clone 19G5 -2586411 2586411
Anti-phosho-erbB2/Her2 (Tyr1112), clone 19G5 -2617718 2617718
Anti-phosho-erbB2/Her2 (Tyr1112), clone 19G5 -2638898 2638898
Anti-phosho-erbB2/Her2 (Tyr1112), clone 19G5 -2817742 2817742
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References

Reference overviewPub Med ID
Tumor-inhibitory antibodies to HER-2/ErbB-2 may act by recruiting c-Cbl and enhancing ubiquitination of HER-2.
Klapper, L N, et al.
Cancer Res., 60: 3384-8 (2000)  2000

Show Abstract 		10910043 10910043

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Categories

Life Science Research > Antibodies and Assays > Primary Antibodies