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OP29 Anti-p53 (Ab-3) (Mutant) Mouse mAb (PAb240)

OP29
Purchase on Sigma-Aldrich

Overview

Key Spec Table

Species ReactivityHostAntibody Type
B, Ch, Ht, H, M, RMMonoclonal Antibody

Products

Catalogue NumberPackaging Qty/Pack
OP29-100UG Plastic ampoule 100 μg
OP29-200UG Plastic ampoule 200 μg
Description
OverviewRecognizes the ~53 kDa mutant p53 protein under non-denaturing conditions by immunoprecipitation, immunofluorescence, and flow cytometry. Recognizes both mutant and wild-type p53 by immunoblotting and paraffin sections under denaturing conditions.
Catalogue NumberOP29
Brand Family Calbiochem®
References
ReferencesEl-Deiry, W.S., et al. 1994. Cancer Res. 54, 1169.
Greenblatt, M.S., et al. 1994. Cancer Res. 54, 4855.
Barak, Y., et al. 1993. EMBO J. 12, 461.
Kastan, M.B., et al. 1992. Cell 71, 587.
Kuerbitz, S.J. 1992. Proc. Natl. Acad. Sci. USA 89, 7491.
Lane, D.P. 1992. Nature 358, 15.
Kastan, M.B., et al. 1991. Cancer Res. 51, 6304.
Product Information
FormLiquid
FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
Negative controlSK-OV-3 cells
Positive controlA431, Hs27 (wild-type p53), or SK-BR-3 cells or breast carcinoma tissue
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Application ReferencesParaffin Sections Pezzella, F., et al. 1994. J. Clin. Pathol. 47, 592. Original Clone Gannon, J.V., et al. 1990. EMBO J. 9, 1595. Epitope Identification Stephen, C.W. and Lane, D.P., 1992. J. Mol. Biol. 225, 1. Immunofluorescence Bernardi, R., et al. 2004. Nat. Cell Biol. 6, 665.
Key Applications Flow Cytometry
Frozen Sections
Gel Shift
Immunoblotting (Western Blotting)
Immunofluorescence
Immunoprecipitation
Paraffin Sections
Application NotesFlow Cytometry (1-20 µg/ml)
Frozen Sections (10 µg/ml)
Gel Shift (see comments)
Immunoblotting (5 µg/ml)
Immunofluorescence (1-20 µg/ml, see application references)
Immunoprecipitation (1 µg per sample)
Paraffin Sections (see application references)
Application CommentsUnder non-denaturing conditions (immunoprecipitation, immunofluorescence and frozen sections), Anti-p53 (Ab-3) does not recognize normal (wild-type) p53 protein; it recognizes an epitope exposed by activating mutations or denaturation. In denaturing protocols (immunoblotting and paraffin sections), Anti-p53 (Ab-3) will recognize both mutant and wild-type p53. Will not recognize to some p53 molecules with mutations in the RHSVV epitope, but will react to TFIIIA, which has the RHSVV epitope. For gel shift assay, use Cat. No. OP29L and resuspend in 100 µl buffer. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogena recombinant protein consisting of amino acids 14-389 of p53 fused to β-galactosidase
ImmunogenHuman
Epitopewithin amino acids 213-217
ClonePAb240
HostMouse
IsotypeIgG₁
Species Reactivity
  • Bovine
  • Chicken
  • Hamster
  • Human
  • Mouse
  • Rat
Antibody TypeMonoclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Yes
Global Trade Item Number
Catalogue Number GTIN
OP29-100UG 07790788053888
OP29-200UG 04055977208429