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ABE2695 Anti-eIF-4E

Anti-eIF-4E, Cat. No. ABE2695, is a highly specific rabbit polyclonal antibody that targets Eukaryotic translation initiation factor 4E and has been tested in Immunocytochemistry and Western Blotting.

Anti-eIF-4E MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Gene Symbol: EIF4E, EIF4EL1, EIF4F UniProt Number: P06730
ABE2695
100 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
M, R, HWB, ICCRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABE2695
DescriptionAnti-eIF-4E
Alternate Names
  • Eukaryotic translation initiation factor 4E
  • eIF4E
  • eIF-4F 25 kDa subunit
  • mRNA cap-binding protein
Background InformationEukaryotic translation initiation factor 4E (UniProt: P06730; also known as eIF-4E, eIF4E, eIF-4F 25 kDa subunit, mRNA cap-binding protein) is encoded by the EIF4E (also known as EIF4EL1, EIF4F) gene (Gene ID: 1977) in human. elF-4E is a cytoplasmic factor that recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. It is a component of the CYFIP1-EIF4E-FMR1 complex that binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex. Three isoforms of elf-4E have been described that are generated by alternative splicing. Elf-4E contains four 7-methylguanosine-containing mRNA cap binding regions and three EIF4EBP1/2/3 binding regions. Hypophosphorylated EIF4EBP1, EIF4EBP2, and EIF4EBP3 are shown to compete with EIF4G1/EIF4G3 to interact with elF-4E. Phosphorylation of ElF4EBP1 by insulin-stimulated MAP kinase (MAPK1 and MAPK3) cause dissociation of the complex and allows EIF4G1/EIF4G3 to bind and result in initiation of translation. Mutations in ElF4E gene have been linked to autism with impairments in social interaction and communication and developmental abnormalities.
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-eIF-4E, Cat. No. ABE2695, is a highly specific rabbit polyclonal antibody that targets Eukaryotic translation initiation factor 4E and has been tested in Immunocytochemistry and Western Blotting.
Key Applications
  • Western Blotting
  • Immunocytochemistry
Application NotesWestern Blotting Analysis: 0.9 µg/mL from a representative lot detected eIF-4E in 10 µg of PC12 cell lysate.

Immunocytochemistry Analysis: A 1:100-500 dilution from a representative lot detected eIF-4E in HeLa, A431, HUVEC, and NIH/3T3 cell lines.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to 14 amino acids from the N-terminal region of human Eukaryotic translation initiation factor 4E.
Epitopeunknown
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis rabbit polyclonal antibody detects Eukaryotic translation initiation factor 4E in Human, Mouse, and Rat cells. it targets an epitope with in 14 amino acids from the N-terminal region.
IsotypeIgG
Species Reactivity
  • Mouse
  • Rat
  • Human
Species Reactivity NoteHuman, Mouse, Rat. Predicted to react with Monkey based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • EIF4E
  • EIF4EL1
  • EIF4F
Purification MethodAffinity Purified
UniProt Number
Molecular Weight~31 kDa observed; 25.10 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in Jurkat cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected eIF-4E in 10 µg of Jurkat cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABE2695 04054839324321