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MAB2610 Anti-Zyxin Antibody, clone 14E11.1

This Anti-Zyxin Antibody, clone 14E11.1 is validated for use in WB, IC for the detection of Zyxin.

Anti-Zyxin Antibody, clone 14E11.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Research Sub-Category: Cytoskeletal Signaling Gene Symbol: ESP-2, HED-2, ZYX UniProt Number: Q15942
MAB2610
100 µg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, R, MWB, ICCMPurifiedMonoclonal Antibody
Description
Catalogue NumberMAB2610
DescriptionAnti-Zyxin Antibody, clone 14E11.1
Alternate Names
  • Zyxin2
  • Zyxin
  • Zyxin-2
Background InformationZyxin, is a cytosolic, focal adhesion protein belonging to the Ajuba family and containing 3 LIM zinc-binding domains. It associates with the actin cytoskeleton near the adhesion plaques and, during mitosis, with microtubules. Zyxin localizes to stress fibers, cell-cell junctions and colocalizes with vinculin, paxillin and VASP at focal adhesions but not at the tips of lamellipodia. Zyxin is involved in the regulation of actin dynamics and is a likely component of the signal transduction pathway mediating adhesion-stimulated changes in gene expression. Zyxin displays an actin nucleating activity that is dependent on its amino terminal proline-rich motifs and independent of Arp2/3 complex. Zyxin appears to function as an adaptor or docking protein to organize protein complexes important for regulated actin cytoskeleton dynamics.
References
Product Information
FormatPurified
Control
  • HUVEC cell lysate
PresentationPurified mouse monoclonal IgMκ in buffer containing PBS with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-Zyxin Antibody, clone 14E11.1 is validated for use in WB, IC for the detection of Zyxin.
Key Applications
  • Western Blotting
  • Immunocytochemistry
Application NotesImmunocytochemistry Analysis: A 1:500 dilution from a previous lot detected Zyxin in NIH/3T3, A431, and HeLa cells.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to human Zyxin.
Clone14E11.1
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
IsotypeIgMκ
Species Reactivity
  • Human
  • Rat
  • Mouse
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryFocal adhesions are actin-rich structures that enable cells to adhere to the extracellular matrix and at which protein complexes involved in signal transduction assemble. Zyxin is a zinc-binding phosphoprotein that concentrates at focal adhesions and along the actin cytoskeleton. Zyxin has an N-terminal proline-rich domain and three LIM domains in its C-terminal half. The proline-rich domain may interact with SH3 domains of proteins involved in signal transduction pathways while the LIM domains are likely involved in protein-protein binding. Zyxin may function as a messenger in the signal transduction pathway that mediates adhesion-stimulated changes in gene expression and may modulate the cytoskeletal organization of actin bundles. Alternative splicing results in multiple transcript variants that encode the same isoform. [provided by RefSeq].
Gene Symbol
  • ESP-2
  • HED-2
  • ZYX
UniProt Number
UniProt SummaryFUNCTION: Adhesion plaque protein. Binds alpha-actinin and the CRP protein. Important for targeting TES and ENA/VASP family members to focal adhesions and for the formation of actin-rich structures. May be a component of a signal transduction pathway that mediates adhesion-stimulated changes in gene expression By similarity.

SUBUNIT STRUCTURE: Interacts with HPV type 6 protein E6. Does not interact significantly with E6 proteins from HPV types 11, 16, or 18. Interacts, via the Pro-rich regions, with the EVH1 domains of ENAH, EVL and VASP. Interacts with the first LIM domain of TES.

SUBCELLULAR LOCATION: Cytoplasm. Cytoplasm › cytoskeleton. Nucleus. Cell junction › focal adhesion. Note: Associates with the actin cytoskeleton near the adhesion plaques. Enters the nucleus in the presence of HESX1.

SEQUENCE SIMILARITIES: Belongs to the zyxin/ajuba family.

Contains 3 LIM zinc-binding domains.
Molecular Weight~84 kDa observed. A non-specific band may be observed at ~36 kDa.
The calculated molecular weight is 61 kDa. Zyxin is a phosphoprotein with an apparent molecular weight of 82–84 kDa (van der Gaag, E., et al. (2002). Journal of Investigative Dermatology. 118:246–254.). As a substrate of caspases, Zyxin can be cleaved as 30, 37, 48 and 70 kDa. (C-B Chan, C-B., et al. (2007). Cell Death and Differentiation. 14:1688–1699.)
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in HUVEC cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected Zyxin in 10 µg of HUVEC cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MAB2610 04053252291104